FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75892
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC970.12781320824329312No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC840.11068360301481053No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC810.10673061719285301No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10673061719285301No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.10673061719285301No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC790.10409529331154797No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10277763137089549No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.10277763137089549No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCT108.4494747E-4295.000031
AGATCCT108.4494747E-4295.000031
CCTAGGA108.4494747E-4295.000035
TAGACCT108.4494747E-4295.000031
GTCCTCG108.4494747E-4295.000033
GGATCCT400.0295.000031
CGCTCCT400.0295.000031
GGGCCTA800.0295.000032
GCCTAGG206.147093E-8295.000034
TACTCCT400.0295.000031
ACGTCCT400.0295.000031
AGCCTAG108.4494747E-4295.000033
GGCGCCT206.147093E-8295.000031
GCGTCCT400.0295.000031
ATCGCCT206.147093E-8295.000031
TAGTCCT206.147093E-8295.000031
CGGACCT400.0295.000031
TGATCCT157.2137864E-6295.01
TCGCCCT350.0295.01
CCCCTAC4450.0295.03
Produced by FastQC (version 0.11.5)