FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52582
Sequences flagged as poor quality0
Sequence length301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA33146.302536989844433No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA23574.482522536229128No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA17753.3756798904568104No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA17523.3319386862424403No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA16343.107527290707847No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA14342.7271689931915866No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13742.6130615039367084No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12162.3125784488988628No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12122.3049712829485376No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10351.9683541896466472No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9401.7876839983264237No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8671.6488532197329886No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8101.5404511049408542No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7911.5043170666768095No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7721.4681830284127648No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7611.4472633220493705No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7301.3883077859343502No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7231.374995245521281No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6991.3293522498193298No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6071.15438743296185No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5391.0250656118063215No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5361.0193602373435777No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5230.9946369480050208No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5090.9680118671788825No Hit
GGACTACAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5050.9604047012285573No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4260.8101631737096345No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4030.7664219694952645No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4020.7645201780076832No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3760.7150735993305694No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3400.6466091057776425No Hit
GGACTAAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3350.637100148339736No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3210.6104750675135978No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3210.6104750675135978No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3200.6085732760260165No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3190.6066714845384352No Hit
GGACTACCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2770.5267962420600205No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2770.5267962420600205No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2750.5229926590848579No Hit
GGACTACAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2490.47354608040774415No Hit
GGACTACGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2350.4469209995816059No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2280.4336084591685368No Hit
GGACTAACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2090.397474420904492No Hit
GGACTAAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG2040.3879654634665855No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2010.3822600890038416No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2010.3822600890038416No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1970.3746529230535164No Hit
GGACTATAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1930.3670457571031912No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1870.35563500817770344No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1860.3537332166901221No Hit
GGACTACAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1810.34422425925221556No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1770.3366170933018904No Hit
GGACTACCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1720.32710813586398385No Hit
GGACTAAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1720.32710813586398385No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1680.3195009699136587No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1600.30428663801300826No Hit
GGACTACGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1530.29097409759993914No Hit
GGACTAACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1520.28907230611235785No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1500.28526872313719526No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1480.28146514016203267No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1430.27195618272412614No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1420.2700543912365448No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1420.2700543912365448No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1400.2662508082613822No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1300.24723289338556922No Hit
GGACTATGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1290.2453311018979879No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.23582214446008137No Hit
GGACTACCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1200.2282149785097562No Hit
GGACTAAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1180.22441139553459358No Hit
GGACTACAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1160.220607812559431No Hit
GGACTATCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1150.21870602107184967No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1080.20539348065878055No Hit
GGACTACAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1040.19778631470845537No Hit
GGACTAAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1030.19588452322087407No Hit
GGACTACGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1020.19398273173329275No Hit
GGACTAAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA1010.19208094024571146No Hit
GGACTATAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA990.18827735727054887No Hit
GGACTATAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG950.18067019132022363No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA930.17686660834506104No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA900.17116123388231716No Hit
GGACTAACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA900.17116123388231716No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA890.16925944239473584No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA860.16355406793199193No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA850.16165227644441063No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA810.15404511049408542No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.15404511049408542No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA790.1502415275189228No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA780.1483397360313415No Hit
GGACTAAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA770.14643794454376022No Hit
GGACTATGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG740.14073257008101633No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA730.138830778593435No Hit
GGACTACGGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG700.1331254041306911No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA690.1312236126431098No Hit
GGACTACGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA680.1293218211555285No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAACATCA670.12742002966794722No Hit
GGACTACCGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG670.12742002966794722No Hit
GGACTATGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA660.12551823818036592No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA650.12361644669278461No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.12361644669278461No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA650.12361644669278461No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA650.12361644669278461No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA640.1217146552052033No Hit
GGACTATCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA630.119812863717622No Hit
GGACTAACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA620.11791107223004069No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.11791107223004069No Hit
GGACTACCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA610.11600928074245939No Hit
GGACTAAAGGGTCTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG580.1103039062797155No Hit
GGACTACAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG550.1045985318169716No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA550.1045985318169716No Hit
GGACTACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGC540.10269674032939027No Hit
GGACTACTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG530.10079494884180898No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA530.10079494884180898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAT1850.0295.280031
GGACTAA2800.0295.280031
GGACTAC43550.0295.281
AGGGGTC255.2386895E-10294.999057
ACTCGGG3300.0294.999056
CTGGGTA108.4421784E-4294.999057
CGGGGTC206.129267E-8294.999057
TAACGGG800.0294.999055
TATCGGG550.0294.999055
CTAGGGT4400.0294.999057
CAGGGTC108.4421784E-4294.999057
TACAAGG7750.0294.999055
ATCGGGT550.0294.999056
CTAACGG800.0294.999054
TACTGGG2750.0294.999055
TGTAGCC108.4421784E-4294.99905295
ATTTGCC108.4421784E-4294.99905295
TGGGTAT108.4421784E-4294.999058
ACTAGGG4400.0294.999056
GGGGTCT500.0294.999058
Produced by FastQC (version 0.11.5)