FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54633
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTC550.10067175516629144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCT350.0295.000031
TCACCCT350.0295.000031
CAGCCTA700.0295.000032
ATCCCTA350.0295.000032
GCCCTAC2800.0295.000033
ACGTCCT350.0295.000031
TTGCCTA700.0295.000032
GTGCCTA350.0295.000032
TCTGCCT157.2027287E-6295.01
AATCCTA650.0295.02
AGTCCTA1100.0295.02
TTGGCCT303.6379788E-12295.01
ATCTCCT303.6379788E-12295.01
ACGCCTA750.0295.02
TTAGCCT255.2386895E-10295.01
AGCCCTA900.0295.02
GACACCT303.6379788E-12295.01
GGGTCCT157.2027287E-6295.01
ACACCTA1150.0295.02
GATCCTA750.0295.02
Produced by FastQC (version 0.11.5)