FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68819
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1290.18744823377265No Hit
TTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1100.1598395791859806No Hit
CTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1060.15402723085194497No Hit
TCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1050.15257414376843606No Hit
ACAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1040.15112105668492715No Hit
TTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1010.14676179543440038No Hit
TCCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1000.14530870835089146No Hit
TTTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC990.14385562126738255No Hit
CCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC990.14385562126738255No Hit
ATATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC990.14385562126738255No Hit
TTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC990.14385562126738255No Hit
ATCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC990.14385562126738255No Hit
TTGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC980.14240253418387364No Hit
CACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC960.1394963600168558No Hit
AACCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC960.1394963600168558No Hit
CTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC930.13513709876632907No Hit
AATTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC930.13513709876632907No Hit
TTACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC930.13513709876632907No Hit
TCCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC920.13368401168282015No Hit
ACATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC910.13223092459931124No Hit
CATTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC900.13077783751580233No Hit
TTTCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC890.1293247504322934No Hit
TTGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC890.1293247504322934No Hit
TATACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC890.1293247504322934No Hit
AAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC890.1293247504322934No Hit
ACTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC880.1278716633487845No Hit
TCACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC880.1278716633487845No Hit
GGCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC880.1278716633487845No Hit
TTGGCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC880.1278716633487845No Hit
TAGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC880.1278716633487845No Hit
TCCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC870.12641857626527558No Hit
ACCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC870.12641857626527558No Hit
TCTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC860.12496548918176666No Hit
ATTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC860.12496548918176666No Hit
ATCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC860.12496548918176666No Hit
CAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC850.12351240209825774No Hit
TAACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC850.12351240209825774No Hit
CCTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC850.12351240209825774No Hit
CACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC850.12351240209825774No Hit
TTCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC840.12205931501474883No Hit
AGTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC840.12205931501474883No Hit
TGATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC840.12205931501474883No Hit
ATACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC840.12205931501474883No Hit
CCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC840.12205931501474883No Hit
CATACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC830.12060622793123991No Hit
TACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC830.12060622793123991No Hit
GCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC820.11915314084773101No Hit
TAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC820.11915314084773101No Hit
TTCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC820.11915314084773101No Hit
CTCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC820.11915314084773101No Hit
AAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC810.1177000537642221No Hit
TTTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC810.1177000537642221No Hit
TGAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC800.11624696668071319No Hit
CCTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC800.11624696668071319No Hit
ATAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.11479387959720426No Hit
CAGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.11479387959720426No Hit
ATTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.11479387959720426No Hit
AGGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.11479387959720426No Hit
TGCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.11479387959720426No Hit
TACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC790.11479387959720426No Hit
GTTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC780.11334079251369535No Hit
CAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC770.11188770543018643No Hit
CGATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC770.11188770543018643No Hit
CTGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC770.11188770543018643No Hit
CTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC770.11188770543018643No Hit
CCCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC760.11043461834667752No Hit
AAGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC760.11043461834667752No Hit
CTTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC760.11043461834667752No Hit
ACGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC760.11043461834667752No Hit
CCCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC760.11043461834667752No Hit
GTACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC750.1089815312631686No Hit
TGCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC750.1089815312631686No Hit
ATCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC740.10752844417965968No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC740.10752844417965968No Hit
TTGCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC740.10752844417965968No Hit
TGACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC740.10752844417965968No Hit
ATGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.10607535709615076No Hit
TAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.10607535709615076No Hit
TATCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.10607535709615076No Hit
AATACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.10607535709615076No Hit
GGGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.10607535709615076No Hit
AACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.10607535709615076No Hit
CTTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC730.10607535709615076No Hit
GACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC720.10462227001264185No Hit
AGGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC720.10462227001264185No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC720.10462227001264185No Hit
GAACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC720.10462227001264185No Hit
TGTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC720.10462227001264185No Hit
GTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC720.10462227001264185No Hit
CATCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.10316918292913294No Hit
GTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.10316918292913294No Hit
GTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC710.10316918292913294No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC710.10316918292913294No Hit
CCGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.10171609584562402No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC700.10171609584562402No Hit
TACCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.10171609584562402No Hit
AACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.10171609584562402No Hit
ACTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.10171609584562402No Hit
AAGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.10171609584562402No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC700.10171609584562402No Hit
CTTCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC700.10171609584562402No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC690.10026300876211512No Hit
CTCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC690.10026300876211512No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC690.10026300876211512No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC690.10026300876211512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCTA850.0295.000032
CTGCCTA550.0295.000032
ATCCCTA850.0295.000032
TCGTCCT550.0295.000031
GTCCCTA550.0295.000032
GAACCTA850.0295.000032
TCCCCTA850.0295.000032
CGTCCTA1550.0295.000032
TCGCCCT206.142909E-8295.01
TGCCCCT108.447771E-4295.01
TACCACG108.447771E-4295.03
GTTTCCT206.142909E-8295.01
CAGCCTA1150.0295.02
GAGTCCT255.2568794E-10295.01
GCGCGAA108.447771E-4295.0295
GTCCTAC4550.0295.03
CTCCCTA750.0295.02
GGTCCTA1050.0295.02
TTGTCCT350.0295.01
CACGCCT350.0295.01
Produced by FastQC (version 0.11.5)