FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117933
Sequences flagged as poor quality0
Sequence length301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1730.14669346154172286No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1660.14075788795332944No Hit
ACATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1640.13906200978521704No Hit
TTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1630.13821407070116085No Hit
TCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1620.13736613161710465No Hit
TCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1590.13482231436493602No Hit
TCCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1560.13227849711276743No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1560.13227849711276743No Hit
TTCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1550.13143055802871123No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1550.13143055802871123No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1530.12973467986059883No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1530.12973467986059883No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1530.12973467986059883No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1530.12973467986059883No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1530.12973467986059883No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1520.1288867407765426No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1510.1280388016924864No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1490.126342923524374No Hit
TTTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1480.1254949844403178No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1480.1254949844403178No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1470.1246470453562616No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1460.1237991062722054No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1430.12125528902003681No Hit
TAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1430.12125528902003681No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1430.12125528902003681No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1430.12125528902003681No Hit
TAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1420.12040734993598061No Hit
TTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1420.12040734993598061No Hit
TTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1420.12040734993598061No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1410.11955941085192441No Hit
ACCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1400.11871147176786818No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1390.11786353268381199No Hit
TCTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1380.11701559359975579No Hit
CTAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1380.11701559359975579No Hit
TTCCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1380.11701559359975579No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1380.11701559359975579No Hit
GGAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1380.11701559359975579No Hit
ATCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1370.11616765451569959No Hit
TCACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1370.11616765451569959No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1370.11616765451569959No Hit
TCCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1360.11531971543164339No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1360.11531971543164339No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1360.11531971543164339No Hit
TTACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1350.11447177634758719No Hit
TTTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1340.113623837263531No Hit
CAATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1330.1127758981794748No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1330.1127758981794748No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1330.1127758981794748No Hit
CTATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1330.1127758981794748No Hit
GTCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1320.1119279590954186No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1320.1119279590954186No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1320.1119279590954186No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1320.1119279590954186No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1310.11108002001136238No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1310.11108002001136238No Hit
ACAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1310.11108002001136238No Hit
CCAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1300.11023208092730619No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1300.11023208092730619No Hit
ACGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1300.11023208092730619No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1290.10938414184324997No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1290.10938414184324997No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1290.10938414184324997No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1290.10938414184324997No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1290.10938414184324997No Hit
ATTACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1280.10853620275919378No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1270.10768826367513758No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1270.10768826367513758No Hit
ATACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1270.10768826367513758No Hit
CATACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1270.10768826367513758No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1270.10768826367513758No Hit
CCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1270.10768826367513758No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1270.10768826367513758No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1270.10768826367513758No Hit
ATAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1260.10684032459108138No Hit
CAAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1260.10684032459108138No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1260.10684032459108138No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1260.10684032459108138No Hit
CCCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1260.10684032459108138No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1250.10599238550702518No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1250.10599238550702518No Hit
AACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1250.10599238550702518No Hit
ACTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1250.10599238550702518No Hit
TTTCCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1250.10599238550702518No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1250.10599238550702518No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1240.10514444642296897No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1240.10514444642296897No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1240.10514444642296897No Hit
CACACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1240.10514444642296897No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1230.10429650733891277No Hit
ACACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1230.10429650733891277No Hit
CAACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1230.10429650733891277No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1230.10429650733891277No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1230.10429650733891277No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1230.10429650733891277No Hit
ATCACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1230.10429650733891277No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1230.10429650733891277No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1220.10344856825485657No Hit
TACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1220.10344856825485657No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1220.10344856825485657No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1220.10344856825485657No Hit
AGGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1220.10344856825485657No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1210.10260062917080037No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1210.10260062917080037No Hit
CACTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1210.10260062917080037No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1210.10260062917080037No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1210.10260062917080037No Hit
TCGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1210.10260062917080037No Hit
ATATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1200.10175269008674417No Hit
TTGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1200.10175269008674417No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1200.10175269008674417No Hit
CTTTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1200.10175269008674417No Hit
TTGACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1200.10175269008674417No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1190.10090475100268796No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1190.10090475100268796No Hit
TATACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1190.10090475100268796No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCC1190.10090475100268796No Hit
GTACCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1190.10090475100268796No Hit
TGATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1190.10090475100268796No Hit
CTCTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1190.10090475100268796No Hit
TAGTCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC1190.10090475100268796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1550.0295.000032
CCCCCTA1550.0295.000032
TGATCCT400.0295.01
CCTTCCT950.0295.01
GCCCCTA1100.0295.02
CGGAAGG108.455412E-4295.09
AATCCTA2250.0295.02
GATACCT350.0295.01
ATCTCCT850.0295.01
TGCCCTA1500.0295.02
AGATCCT500.0295.01
GGAGCCT255.275069E-10295.01
TCACCCT650.0295.01
AGCCCTA1600.0295.02
GAGTCCT550.0295.01
CCCTAGG206.161463E-8295.04
CCCTACA108.455412E-4295.04
GGGTCCT350.0295.01
CGGCCCT255.275069E-10295.01
GATCCTA2250.0295.02
Produced by FastQC (version 0.11.5)