FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83770
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA15131.8061358481556644No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA13531.6151366837770087No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA10471.2498507819028293No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA10381.2391070789065297No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10321.2319446102423302No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10081.2032947355855317No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA9641.1507699653814014No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA9621.1483824758266683No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA9281.1077951533962038No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA9201.0982451951772711No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA8040.9597708010027456No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA8000.9549958218932793No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7820.9335084159006805No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7680.916795989017548No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7480.8929210934702161No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7400.8833711352512832No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7260.866658708368151No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA7210.8606899844813178No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA7130.851140026262385No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7020.8380088337113525No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7000.8356213441566193No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6730.8033902351677211No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA6620.7902590426166886No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6510.777127850065656No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA6230.7437029962993912No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6160.7353467828578251No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6040.7210218455294258No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA5950.7102781425331264No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA5940.7090843977557598No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA5930.7078906529783933No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA5900.7043094186462934No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5760.687596991763161No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5730.6840157574310612No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5660.675659543989495No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA5630.6720783096573952No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA5530.6601408618837292No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5480.6541721379968963No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5230.6243285185627312No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5190.6195535394532649No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5170.6171660498985317No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA5090.6076160916795988No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA4930.5885161752417333No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4910.5861286856870002No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4850.5789662170228005No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA4810.5741912379133342No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4760.5682225140265011No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA4730.5646412796944014No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4590.5479288528112689No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA4530.5407663841470693No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4530.5407663841470693No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4530.5407663841470693No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4490.5359914050376029No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA4430.5288289363734034No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4390.524053957263937No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4380.5228602124865703No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4370.5216664677092038No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4140.49421033782977203No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4060.48466037961083924No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3930.4691416975050734No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3920.4679479527277068No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3890.46436671839560706No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3870.4619792288408738No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA3770.4500417810672079No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3740.44646054673510804No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3640.43452309896144203No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA3620.43213560940670886No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3500.41781067207830963No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3480.41542318252357646No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3430.4094544586367435No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3350.3999045004178106No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3320.3963232660857109No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA3320.3963232660857109No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3300.39393577653097767No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA3290.3927420317536111No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3270.3903545421988779No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3200.38199832875731166No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA3170.37841709442521193No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA3140.3748358600931121No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3070.3664796466515459No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA3050.3640921570968127No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2970.3545421988778799No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2970.3545421988778799No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2930.3497672197684135No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2800.3342485376626477No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2720.32469857944371494No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2710.32350483466634833No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2680.31992360033424855No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA2670.31872985555688194No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2650.3163423660021487No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2650.3163423660021487No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2630.31395487644741554No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2620.31276113167004893No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2610.3115673868926824No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2580.30798615256058254No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2560.3055986630058493No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2520.3008236838963829No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA2490.29724244956428314No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA2480.2960487047869166No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA2440.2912737256774502No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA2410.28769249134535035No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2370.28291751223588396No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2330.27814253312641757No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2320.27694878834905096No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2300.2745612987943178No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2220.265011340575385No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2180.2602363614659186No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2170.259042616688552No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2100.2506864032469858No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2020.24113644502805298No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2000.2387489554733198No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1980.23636146591858662No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1980.23636146591858662No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1970.23516772114122003No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1960.2339739763638534No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1950.2327802315864868No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1950.2327802315864868No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1940.23158648680912022No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1900.22681150769965383No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1870.22323027336755402No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1750.20890533603915482No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1720.205324101707055No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1720.205324101707055No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1680.20054912259758864No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1660.19816163304285545No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1610.19219290915602244No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1570.18741793004655605No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1560.18622418526918944No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1550.18503044049182285No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1530.18264295093708965No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1500.17906171660498985No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1440.17189924794079026No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.17070550316342364No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1410.16831801360869045No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1350.16115554494449089No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1310.15638056583502447No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1310.15638056583502447No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTTA1290.15399307628029127No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1260.15041184194819146No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1260.15041184194819146No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1230.14683060761609168No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1230.14683060761609168No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1150.1372806493971589No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1150.1372806493971589No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1110.1325056702876925No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1110.1325056702876925No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1090.1301181807329593No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1090.1301181807329593No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1080.1289244359555927No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA1070.1277306911782261No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1030.12295571206875969No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTTA1030.12295571206875969No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCTTTA1000.1193744777366599No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1000.1193744777366599No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA990.11818073295929331No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA960.1145994986271935No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGTGCCTCA960.1145994986271935No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG960.1145994986271935No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA930.1110182642950937No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG910.10863077474036051No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG890.10624328518562731No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA880.1050495404082607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAC81150.0295.351441
ACCCGGG6550.0294.998846
CGGGTTC108.451148E-4294.99889
ATTAGCC108.451148E-4294.9988295
ACGGGTA6150.0294.99888
CAGGGTA4000.0294.99888
TGTTAGC206.1510946E-8294.9988295
AGTTCGC400.0294.9988295
CTACCAG9400.0294.99884
TACTCGG8100.0294.99885
ACACGGG12150.0294.998786
CACGGGT12150.0294.998787
TACACGG12200.0293.78985
CAAGGGT15300.0293.07077
GACTACA34500.0292.86112
CTACTAG12300.0292.600434
TACTAGG12300.0292.600435
TAGGGTA5900.0292.49888
AGGGTAT17200.0292.426129
ACAAGGG15350.0292.11616
Produced by FastQC (version 0.11.5)