FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57959
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC590.10179609724115322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT350.0295.000031
AGAACCT350.0295.000031
CGGTCCT350.0295.000031
CGATCCT350.0295.000031
ATACCTA700.0295.000032
GTGCCTA700.0295.000032
TGATCCT206.134724E-8295.01
TCGCCTA800.0295.02
AGTCCTA900.0295.02
GTACCTA900.0295.02
TGCCCCT108.444335E-4295.01
CGAACCT255.2568794E-10295.01
CCCTCGG206.134724E-8295.04
ACGCCCT206.134724E-8295.01
GTCGATA108.444335E-4295.0295
CCCTCCT450.0295.01
GAGTCCT206.134724E-8295.01
CGGCCCT255.2568794E-10295.01
GTCCTAC4100.0295.03
GATCCTA1050.0295.02
Produced by FastQC (version 0.11.5)