FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63097
Sequences flagged as poor quality0
Sequence length301
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA650.0295.000032
GCGCCTA650.0295.000032
TGATCCT157.2080165E-6295.01
GCCCCTA450.0295.02
GCGCGAA157.2080165E-6295.0295
TTGTCCT157.2080165E-6295.01
CGCGCCT303.6379788E-12295.01
TACGGCG157.2080165E-6295.07
CGTTCCT303.6379788E-12295.01
GACCCCT303.6379788E-12295.01
TACGCCT350.0295.01
ACGGCGG157.2080165E-6295.08
CTACGGC255.2568794E-10295.06
ACAGCCT255.2568794E-10295.01
GTTCCCT255.2568794E-10295.01
CTACCTA450.0295.02
CTCGCCT303.6379788E-12295.01
CCGACCT157.2080165E-6295.01
TTGCCTA850.0295.02
GACGCCT157.2080165E-6295.01
Produced by FastQC (version 0.11.5)