FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84125
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1400.1664190193164933No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1350.16047548291233282No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1330.15809806835066864No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1310.15572065378900446No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1270.1509658246656761No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1260.14977711738484398No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1260.14977711738484398No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1230.1462109955423477No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.14502228826151561No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.1438335809806835No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.13670133729569092No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.13670133729569092No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.13551263001485883No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1140.13551263001485883No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.13432392273402674No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.13313521545319465No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.13313521545319465No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.13313521545319465No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.13194650817236256No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.13194650817236256No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.13075780089153047No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.13075780089153047No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.13075780089153047No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.13075780089153047No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.12956909361069838No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.12956909361069838No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.12956909361069838No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.12838038632986626No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.12838038632986626No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.12719167904903417No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.12600297176820208No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.12600297176820208No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.12600297176820208No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.12481426448736999No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.12481426448736999No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.12481426448736999No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.1236255572065379No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.1236255572065379No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.1236255572065379No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1224368499257058No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1224368499257058No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1224368499257058No Hit
ACAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1224368499257058No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1224368499257058No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1224368499257058No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.12124814264487371No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.12005943536404161No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.12005943536404161No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.12005943536404161No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1188707280832095No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1188707280832095No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1188707280832095No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1188707280832095No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1188707280832095No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.1188707280832095No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.11768202080237741No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.11768202080237741No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.11649331352154531No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.11649331352154531No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.11530460624071322No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11411589895988113No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11411589895988113No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11411589895988113No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11411589895988113No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11411589895988113No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.11292719167904904No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.11173848439821694No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.11173848439821694No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.11173848439821694No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11054977711738485No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.11054977711738485No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10936106983655276No Hit
AACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10936106983655276No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10936106983655276No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10936106983655276No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10936106983655276No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10936106983655276No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10936106983655276No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.10817236255572064No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.10698365527488855No Hit
GTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.10698365527488855No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.10698365527488855No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10579494799405646No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10579494799405646No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10579494799405646No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10579494799405646No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10579494799405646No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10579494799405646No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10460624071322437No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10460624071322437No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10460624071322437No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10460624071322437No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10460624071322437No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10460624071322437No Hit
CCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10341753343239227No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10341753343239227No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10341753343239227No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10341753343239227No Hit
TAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10341753343239227No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10341753343239227No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10341753343239227No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10222882615156018No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10222882615156018No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.10222882615156018No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10104011887072809No Hit
GCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10104011887072809No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10104011887072809No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.10104011887072809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTA1400.0295.000032
AGTCCTA1400.0295.000032
TACCCCT350.0295.000031
TCACCTA1550.0295.000032
CCCTCCT350.0295.000031
TCCGCCT350.0295.000031
CAGCCCT350.0295.000031
CAACCTA1550.0295.000032
CGCGCCT350.0295.000031
GCGCCTA950.0295.000032
GTTACCT350.0295.000031
ATTCCCT350.0295.000031
TTCCCTA1400.0295.000032
TTCCCCT350.0295.000031
AGTGCCT350.0295.000031
TACGCCT350.0295.000031
ATCCCCT350.0295.000031
TCGTCCT350.0295.000031
CCCCCCT350.0295.000031
TCTCCTA1550.0295.000032
Produced by FastQC (version 0.11.5)