FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76077
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1030.13538914520814438No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1020.13407468748767695No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.13144577204674213No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC960.12618794116487242No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.11830119484206791No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.11698673712160047No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.11698673712160047No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.11304336396019822No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.11172890623973081No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.11172890623973081No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.11041444851926337No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.11041444851926337No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10778553307832853No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10778553307832853No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.10778553307832853No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10647107535786111No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10647107535786111No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.10647107535786111No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.1051566176373937No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.10384215991692627No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.10252770219645886No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.10252770219645886No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.10252770219645886No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.10252770219645886No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.10121324447599142No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.10121324447599142No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.10121324447599142No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.10121324447599142No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.10121324447599142No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCT350.0295.000031
AAGACCT350.0295.000031
GGATCCT350.0295.000031
CCGTCCT550.0295.000031
GCACCCT350.0295.000031
CGCTCCT350.0295.000031
CACCCTA1400.0295.000032
ATCGCCT350.0295.000031
ACGACCT350.0295.000031
GTGCCTA700.0295.000032
TGATCCT206.147275E-8295.01
GCCCCTA1250.0295.02
GTCGCCT450.0295.01
TGCCCCT255.2568794E-10295.01
AGATCCT255.2568794E-10295.01
TCCGCCT206.147275E-8295.01
CCCTAGG108.449519E-4295.04
GCGCGAA108.449519E-4295.0295
GGCACCT500.0295.01
GCAACCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)