FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65555
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA14492.210357714895889No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12461.9006940736785907No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10671.6276409122111206No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10411.5879795591488062No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9981.5223857829303638No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9441.4400122034932499No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA9091.3866219205247503No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8921.3606894973686219No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8871.3530623140874074No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8441.287468537868965No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8041.2264510716192512No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA7991.2188238883380367No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7891.2035695217756084No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7791.1883151552131799No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA7601.1593318587445656No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7451.1364503089009228No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7201.0983143924948515No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7141.0891617725573945No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6911.054076729463809No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6861.046449546182595No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6781.034246052932652No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6671.0174662497139806No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6280.9579742201205095No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6180.942719853558081No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6090.9289909236518953No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA6090.9289909236518953No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA6040.9213637403706811No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5940.9061093738082526No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5800.8847532606208528No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5790.8832278239646099No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5780.881702387308367No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5630.8588208374647242No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5570.8496682175272672No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5510.8405155975898101No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5460.8328884143085958No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA5400.8237357943711388No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5380.820684921058653No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5330.8130577377774388No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA5120.781023567996339No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5000.7627183281214247No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4960.7566165814964534No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4950.7550911448402104No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4930.7520402715277248No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4860.7413622149340249No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4660.710853481809168No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4640.7078026084966822No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4620.7047517351841965No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4590.7001754252154679No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4560.6955991152467393No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4520.6894973686217679No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4490.6849210586530394No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4420.6742430020593395No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4380.6681412554343681No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4240.6467851422469681No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4230.6452597055907253No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4130.6300053390282968No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4070.6208527190908397No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4060.6193272824345969No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA4010.6117000991533826No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3940.6010220425596827No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3930.5994966059034399No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3870.5903439859659828No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3860.58881854930974No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3810.5811913660285256No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3720.5674624361223399No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3700.5644115628098544No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3630.5537335062161544No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3570.5445808862786973No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3450.5262756464037831No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3340.5094958431851118No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3300.5033940965601403No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3290.5018686599038975No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3190.486614293341469No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3120.475936236747769No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3120.475936236747769No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3100.4728853634352833No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3020.46068187018534057No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2940.4484783769353977No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2940.4484783769353977No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2760.4210205171230265No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2750.41949508046678363No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2680.40881702387308366No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2670.40729158721684083No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2610.39813896727938375No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2580.3935626573106552No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2540.3874609106856838No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2520.38441003737319807No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2410.3676302341545268No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2360.36000305087331247No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2240.3416978109983983No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2140.3264434444359698No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2110.32186713446724124No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2060.314239951186027No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1720.2623751048737701No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1650.25169704828007017No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1430.2181374418427275No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.19830676531157046No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1240.18915414537411335No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1220.18610327206162763No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.1815269620928991No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1170.17847608878041338No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1170.17847608878041338No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1060.16169628556174204No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1050.16017084890549918No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA970.14796735565555638No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.12813667912439936No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.12813667912439936No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA780.11898405918694226No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA770.11745862253069943No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA750.11440774921821371No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA690.10525512928075663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGC108.44686E-4295.0295
GGACTCC255.2568794E-10295.01
GGACACT108.44686E-4295.01
TACACGG7100.0295.05
GGATACT108.44686E-4295.01
GGTTAGC255.2568794E-10295.0295
ACGGGTA2300.0295.08
AGTTGGC108.44686E-4295.0295
TTAGCCC108.44686E-4295.0295
CTACCGG5750.0295.04
ACTACCG5750.0295.03
GGACTAC61850.0294.5231
ACTACCC7800.0293.108983
CTACTAG7750.0293.09684
ACACGGG7150.0292.937076
GACTACC19400.0292.71912
TACCAGG5850.0292.478645
ACCAGGG5850.0292.478646
ACGGGTT4850.0291.958778
GACTACT25500.0291.52942
Produced by FastQC (version 0.11.5)