FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67515
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA42276.260830926460787No Hit
GGACTACAAGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA39835.8994297563504405No Hit
GGACTACTAGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA32334.788565503962083No Hit
GGACTACACGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA31794.708583277790121No Hit
GGACTACTAGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA29664.393097830111827No Hit
GGACTACACGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA29224.327927127305043No Hit
GGACTACCAGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA25393.7606457824187216No Hit
GGACTACAGGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA23993.5532844553062284No Hit
GGACTACCAGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA22933.3962823076353406No Hit
GGACTACTCGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA22303.302969710434718No Hit
GGACTACTCGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA21273.150411019773384No Hit
GGACTACAGGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA20583.0482115085536545No Hit
GGACTACCCGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA18652.762349107605717No Hit
GGACTACCCGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA17352.5697993038584017No Hit
GGACTACTGGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA17022.520921276753314No Hit
GGACTACTGGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA16362.4231652225431386No Hit
GGACTACCGGGGTTTCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA15362.2750499888913573No Hit
GGACTACCGGGGTATCTAAGCCTGTTTGCTCCCCCAGCTTTCGTGATTCA14032.0780567281344884No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1720.25475820188106346No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1670.2473524401984744No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1540.22809745982374285No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA1370.20291787010294007No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1340.19847441309338668No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1270.18810634673776197No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1250.18514404206472634No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1220.18070058505517292No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA1140.16885136636303044No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1120.16588906168999482No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1110.16440790935347702No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA1100.1629267570169592No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1090.1614456046804414No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1090.1614456046804414No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1030.1525586906613345No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1000.1481152336517811No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA930.1377471672961564No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA930.1377471672961564No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA930.1377471672961564No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA930.1377471672961564No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA930.1377471672961564No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA910.13478486262312078No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA900.13330371028660298No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA890.13182255795008518No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA880.13034140561356736No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA870.12886025327704956No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA870.12886025327704956No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA850.12589794860401393No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA840.1244167962674961No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA800.11849218692142488No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA800.11849218692142488No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT800.11849218692142488No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG800.11849218692142488No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA790.11701103458490705No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA790.11701103458490705No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCCAGCTTTCGTGATTCA780.11552988224838925No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA780.11552988224838925No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG760.11256757757535363No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA710.10516181589276456No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT710.10516181589276456No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA700.10368066355624676No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA700.10368066355624676No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA680.10071835888321114No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA680.10071835888321114No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTTG108.4474165E-4295.04
TACTTGG108.4474165E-4295.05
TACCAAG157.210272E-6295.05
TTAGCCG108.4474165E-4295.0295
CGTTAGC500.0295.0295
ACTACTT108.4474165E-4295.03
ACCAGGG6750.0295.06
CTACCAA157.210272E-6295.04
AGTTAGG108.4474165E-4295.0295
ACCCGGG5200.0295.06
GGACCAC108.4474165E-4295.01
ACTGGGG4450.0295.06
GGACTAC64000.0294.769531
AGGGTAT14000.0293.946449
CTAGGGT9600.0293.463567
CGGGTAT9150.0293.387979
ACACGGG8000.0293.156256
CTACCAG6800.0292.830874
GACTACA27100.0292.822882
CTCGGGT6300.0292.658727
Produced by FastQC (version 0.11.5)