FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82091
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCT255.275069E-10295.01
GCGACCT108.4507343E-4294.999971
CAACGGG108.4507343E-4294.999976
GATCCTA1200.0294.999972
CGTTCCT157.2159346E-6294.999971
ACGGCGG108.4507343E-4294.999978
GCCTAGG108.4507343E-4294.999974
CGTGTTG108.4507343E-4294.99997295
GACGCCT157.2159346E-6294.999971
TTACCCT206.150185E-8294.999971
CGATCCT206.150185E-8294.999971
TCCTACG22000.0292.988654
ACCTACG21450.0290.87414
CCTACGG77700.0290.254185
CTACGGG75650.0289.15076
TACGGGA75100.0289.107857
ACGGGAG75650.0287.200938
CCCTACG19200.0286.549474
GTCCTAC5200.0286.49043
GACCTAC5050.0286.237643
Produced by FastQC (version 0.11.5)