FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82091
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC11621.4155023084138334No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC9171.1170530265193503No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC7710.9392016177169239No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7180.8746391200009745No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6310.7686591709200765No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC6050.7369870022292334No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5740.6992240318670744No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5680.6919150698614952No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5550.6760789855160736No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5310.6468431374937569No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5220.6358796944853882No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC5140.6261344118112826No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4870.5932440827861764No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4850.59080776211765No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4810.5859351207805972No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4720.5749716777722284No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4590.5591355934268069No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4430.5396450280785957No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4340.5286815850702269No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC4230.5152818213933318No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG4000.48726413370527827No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC3840.4677735683570672No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3610.43975588066901367No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3490.42513795665785525No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3430.41782899465227613No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3280.39955658963832824No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3210.3910294672984858No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3200.3898113069642226No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3130.38128418462438024No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3110.37884786395585385No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3030.36910258128174833No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2860.348393855599274No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2760.33621225225664203No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2740.33377593158811564No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2620.3191580075769573No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2610.3179398472426941No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2510.3057582439000621No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG2480.30210376289727253No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2020.24606838752116553No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2010.24485022718690233No Hit
GGACTACAAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1690.20586909649048007No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1670.20343277582195368No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1660.20221461548769049No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1660.20221461548769049No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1660.20221461548769049No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1580.19246933281358494No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1480.18028772947095295No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.16688796579405782No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1340.16323348479126823No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1340.16323348479126823No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1340.16323348479126823No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1320.16079716412274184No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1320.16079716412274184No Hit
GGACTACAAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1270.15470636245142586No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1220.14861556078010987No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1190.1449610797773203No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1190.1449610797773203No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1190.1449610797773203No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1180.1437429194430571No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1180.1437429194430571No Hit
GGACTACAAGGGTATCTAACTGAAGGGGCGATTTCCCTTCACATGTCCAC1170.1425247591087939No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1160.1413065987745307No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1150.1400884384402675No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1120.13643395743747794No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.13156131610042512No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1080.13156131610042512No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1050.12790683509763556No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1050.12790683509763556No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1030.12547051442910914No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1020.12425235409484596No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1020.12425235409484596No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1020.12425235409484596No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1000.12181603342631957No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.12181603342631957No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT990.12059787309205638No Hit
GGACTACAGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT980.11937971275779319No Hit
GGACTACAAGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG970.11816155242352999No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC960.1169433920892668No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC960.1169433920892668No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC950.11572523175500359No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.11450707142074039No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA940.11450707142074039No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC930.1132889110864772No Hit
GGACTACTGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC930.1132889110864772No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA920.11207075075221401No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC920.11207075075221401No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.11085259041795081No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT910.11085259041795081No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC900.10963443008368762No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC900.10963443008368762No Hit
GGACTACAGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT870.10597994908089801No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC870.10597994908089801No Hit
GGACTACCGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC860.10476178874663483No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC850.10354362841237164No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT850.10354362841237164No Hit
GGACTACACGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC850.10354362841237164No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC840.10232546807810844No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.10232546807810844No Hit
GGACTACACGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC830.10110730774384524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)