FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74768
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC830.11101005777872887No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC750.10031029317355017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCTA1250.0295.000032
CCGCCTA1250.0295.000032
GACCTAC5500.0295.000033
TGATCCT255.2568794E-10295.01
TCTGCCT500.0295.01
GCCCCTA600.0295.02
ACAACCT303.6379788E-12295.01
ATCCTCG108.44923E-4295.03
GTGCACA108.44923E-4295.0295
GATACCT303.6379788E-12295.01
TTGGCCT400.0295.01
CCCCTAG157.213368E-6295.03
AGATCCT500.0295.01
CGAACCT400.0295.01
GCGACCT206.146547E-8295.01
TCACCCT303.6379788E-12295.01
GGTCGAT108.44923E-4295.0295
GTTTCCT157.213368E-6295.01
CAACGGG108.44923E-4295.06
CAGCCTA850.0295.02
Produced by FastQC (version 0.11.5)