FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62024
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.14671739971623887No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.14349284148071714No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13704372500967366No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13704372500967366No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.12737005030310847No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12575777118534762No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12575777118534762No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12092093383206501No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12092093383206501No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11769637559654327No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.1160840964787824No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11447181736102155No Hit
GTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11285953824326067No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11285953824326067No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1112472591254998No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10963498000773894No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10963498000773894No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10802270088997806No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10802270088997806No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10641042177221721No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10641042177221721No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10479814265445635No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10479814265445635No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10479814265445635No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10479814265445635No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10479814265445635No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10479814265445635No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10318586353669547No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10318586353669547No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10318586353669547No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10318586353669547No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10318586353669547No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1015735844189346No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1015735844189346No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1015735844189346No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1015735844189346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCTA1100.0295.000032
TGACCTA1100.0295.000032
AAACCTA1250.0295.000032
AGACCTA1100.0295.000032
GTGCCTA550.0295.000032
TCGCCTA900.0295.02
GTTGATT255.2568794E-10295.0295
GCCCCTA650.0295.02
TCGCCCT255.2568794E-10295.01
AATCCTA1450.0295.02
AGTCCTA650.0295.02
GATACCT350.0295.01
TCCGCCT255.2568794E-10295.01
TCACCCT255.2568794E-10295.01
CAGCCTA650.0295.02
AGCCCTA950.0295.02
GTCCTAC3150.0295.03
GATCCTA950.0295.02
CTCCCTA750.0295.02
CAACCTA1300.0295.02
Produced by FastQC (version 0.11.5)