FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68722
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC700.10185966648234918No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC690.10040452838974419No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT350.0295.000031
TCCGCCT350.0295.000031
CGTTCCT350.0295.000031
TAGCCCT350.0295.000031
TCGACCT350.0295.000031
ATCGCCT350.0295.000031
GCATCCT350.0295.000031
TCGCCTA1000.0295.02
AGTCCTA1200.0295.02
TGCCCCT108.4477436E-4295.01
CGAACCT255.2568794E-10295.01
ACGCCTA750.0295.02
GCGACCT255.2568794E-10295.01
CGTGCCT157.2108287E-6295.01
AGCCCTA1050.0295.02
GAGTCCT450.0295.01
GACACCT255.2568794E-10295.01
GGCACCT157.2108287E-6295.01
CTGAGAG108.4477436E-4295.0295
CGACCTA1050.0295.02
Produced by FastQC (version 0.11.5)