FastQCFastQC Report
Wed 8 May 2024
EGAF00005492227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62847
Total Bases18.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12729326777730043No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12729326777730043No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.1209286043884354No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1177462726940029No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11615510684678663No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11456394099957037No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11297277515235414No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11297277515235414No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11138160930513788No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11138160930513788No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11138160930513788No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11138160930513788No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10979044345792162No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10979044345792162No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.10979044345792162No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10819927761070536No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10819927761070536No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10660811176348911No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10660811176348911No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC650.10342578006905659No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.1002434483746241No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1002434483746241No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.1002434483746241No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1002434483746241No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1002434483746241No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.1002434483746241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)