FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62847
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12729326777730043No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12729326777730043No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.1209286043884354No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1177462726940029No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11615510684678663No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11456394099957037No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.11297277515235414No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11297277515235414No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11138160930513788No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11138160930513788No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11138160930513788No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC700.11138160930513788No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10979044345792162No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC690.10979044345792162No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.10979044345792162No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.10819927761070536No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10819927761070536No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10660811176348911No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC670.10660811176348911No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10501694591627285No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC650.10342578006905659No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10183461422184033No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.1002434483746241No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1002434483746241No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC630.1002434483746241No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1002434483746241No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.1002434483746241No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.1002434483746241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG108.446017E-4295.03
CTTTCCT550.0295.01
ATCCCCT255.2568794E-10295.01
ACAGCCT206.1387254E-8295.01
AGCCTAC3050.0295.03
AAACCTA1300.0295.02
TGGTCCT450.0295.01
TCCTCGG255.2568794E-10295.04
ACGCCTA850.0295.02
CCAACCT255.2568794E-10295.01
GGGTCCT350.0295.01
GCCTCCT108.446017E-4295.01
CAGTCCT350.0295.01
GTCACCT400.0295.01
CCTACCT206.1387254E-8295.01
ATCTCCT350.0295.01
AGCTCCT206.1387254E-8295.01
TATACCT206.1387254E-8295.01
TTACCTA750.0295.02
TGACCTA1000.0295.02
Produced by FastQC (version 0.11.5)