FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62847
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA15152.4106162585326265No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA12702.0207806259646444No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10271.6341273250910942No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9681.5402485401053352No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9641.5338838767164702No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9291.4781930720639012No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8781.3970436138558724No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8781.3970436138558724No Hit
GGACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA8621.371584960300412No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8311.3222588190367082No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA7451.1854185561761104No Hit
GGACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA7441.183827390328894No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7361.1710980635511639No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7261.1551864050790013No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7191.1440482441484876No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7181.1424570783012713No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7091.128136585676325No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6761.0756281127181886No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6671.0613076200932423No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA6611.051760625009945No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6401.0183461422184035No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6311.0040256495934572No Hit
GGACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA6230.9912963228157271No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5810.9244673572326443No Hit
GGACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA5680.9037822012188331No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5600.891052874441103No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5510.8767323818161566No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5300.8433178990246154No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5240.8337709039413178No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5170.822632743010804No Hit
GGACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA5080.8083122503858577No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA5030.8003564211497765No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4990.7939917577609115No Hit
GGACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA4980.7924005919136952No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4980.7924005919136952No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4890.7780800992887489No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4890.7780800992887489No Hit
GGACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA4770.7589861091221538No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4610.7335274555666937No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4540.72238929463618No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4510.7176157970945312No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4460.7096599678584499No Hit
GGACTACAAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA4370.6953394752335036No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4350.6921571435390711No Hit
GGACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA4280.6810189826085573No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4230.673063153372476No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4190.666698489983611No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4170.6635161582891785No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4130.6571514949003134No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3990.6348751730392859No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3930.6253281779559884No Hit
GGACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA3740.5950960268588795No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3730.5935048610116632No Hit
GGACTACACGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA3710.5903225293172307No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3690.5871401976227982No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3580.5696373733034195No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3580.5696373733034195No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3580.5696373733034195No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3530.5616815440673382No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3510.5584992123729056No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3510.5584992123729056No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3220.5123554028036342No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3200.5091730711092017No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3140.4996260760259042No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3110.49485257848425546No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3100.49326141263703915No Hit
GGACTACCAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA3100.49326141263703915No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3020.48053208585930907No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3010.4789409200120928No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA3000.4773497541648766No Hit
GGACTACAGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA3000.4773497541648766No Hit
GGACTACCCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA2840.45189110060941656No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2830.4502999347622003No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2780.44234410552611897No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2770.4407529396789027No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2710.4312059445956052No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2570.4089296227345776No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2490.3962002959568476No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2490.3962002959568476No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2470.3930179642624151No Hit
GGACTACTCGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA2400.3818798033319013No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2350.37392397409582No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2340.37233280824860376No Hit
GGACTACCGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA2270.36119464731809No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2200.35005648638757614No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2170.3452829888459274No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2100.3341448279154136No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA2070.32937133037376487No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2040.32459783283211613No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2000.3182331694432511No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1920.305503842665521No Hit
GGACTACTGGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA1850.2943656817350072No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1810.2880010183461422No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1800.286409852498926No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1690.26890702817954715No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1580.2514042038601684No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1580.2514042038601684No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1580.2514042038601684No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1510.24026604292965453No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1440.22912788199914075No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1440.22912788199914075No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1250.19889573090203194No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1180.18775756997151813No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1100.17502824319378807No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1080.17184591149935557No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA1060.16866357980492308No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1050.1670724139577068No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1010.1607077505688418No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA980.15593425302719302No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA980.15593425302719302No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA910.14479609209667924No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA870.13843142870781422No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.1352490970133817No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA820.13047559947173296No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA820.13047559947173296No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA800.12729326777730043No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA800.12729326777730043No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA790.1257021019300842No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA780.12411093608286793No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA750.11933743854121916No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA720.11456394099957037No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA700.11138160930513788No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA690.10979044345792162No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA680.10819927761070536No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA670.10660811176348911No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA660.10501694591627285No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA660.10501694591627285No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA650.10342578006905659No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCA650.10342578006905659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGG6850.0295.04
ACTAACG700.0295.03
CTATAGG206.1387254E-8295.04
CTTTAGC255.2568794E-10295.0295
TACAGGG6850.0295.05
ATTAGCC255.2568794E-10295.0295
GGACTAT750.0295.01
GGACTAA1750.0295.01
TAACGGG700.0295.05
ATAGGGT206.1387254E-8295.06
TATCGGG206.1387254E-8295.05
AATTAGA108.446017E-4295.0295
CTAGGTT108.446017E-4295.07
ATCGGGT206.1387254E-8295.06
CTAACGG700.0295.04
AAAGGGT550.0295.06
TACTGGG4200.0295.05
GGTAGCC108.446017E-4295.0295
ACTAGGT108.446017E-4295.06
ACAGGGT255.2568794E-10295.06
Produced by FastQC (version 0.11.5)