FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71531
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1330.18593337154520417No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1220.17055542352266848No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1200.16775943297311655No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1170.16356544714878862No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1140.15937146132446073No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1120.15657547077490877No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1100.15377948022535684No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1090.15238148495058088No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1040.14539150857670102No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1040.14539150857670102No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.14399351330192503No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1030.14399351330192503No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.14259551802714907No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1010.1411975227523731No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1010.1411975227523731No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.13979952747759714No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.13979952747759714No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.13840153220282114No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.13840153220282114No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC990.13840153220282114No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.13700353692804518No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.1356055416532692No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.1356055416532692No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.13280955110371728No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.13280955110371728No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC950.13280955110371728No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC940.1314115558289413No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12861556527938936No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12861556527938936No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12861556527938936No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12861556527938936No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.1272175700046134No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.1272175700046134No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.1258195747298374No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.1258195747298374No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.1258195747298374No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12442157945506144No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12302358418028547No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12302358418028547No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12302358418028547No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12162558890550951No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12162558890550951No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12162558890550951No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.12162558890550951No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.12022759363073353No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.12022759363073353No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.12022759363073353No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.12022759363073353No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11882959835595756No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11882959835595756No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11882959835595756No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11743160308118158No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11743160308118158No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11743160308118158No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11743160308118158No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11743160308118158No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11463561253162964No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11463561253162964No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11463561253162964No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.11463561253162964No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11323761725685368No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11323761725685368No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11323761725685368No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11323761725685368No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11323761725685368No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11323761725685368No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11183962198207771No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11183962198207771No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11183962198207771No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.11183962198207771No Hit
GAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.11183962198207771No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11044162670730173No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.11044162670730173No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10904363143252577No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10904363143252577No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10904363143252577No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10904363143252577No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10904363143252577No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10764563615774979No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10764563615774979No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10764563615774979No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10764563615774979No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10764563615774979No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10764563615774979No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10624764088297382No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10624764088297382No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10624764088297382No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10624764088297382No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10624764088297382No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10484964560819784No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.10484964560819784No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10345165033342188No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10345165033342188No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10345165033342188No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10345165033342188No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10345165033342188No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10345165033342188No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10345165033342188No Hit
GGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10205365505864591No Hit
CGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10205365505864591No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10205365505864591No Hit
AGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10205365505864591No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10065565978386994No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10065565978386994No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10065565978386994No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10065565978386994No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC720.10065565978386994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCT550.0295.000031
TTACCCT550.0295.000031
TGATCCT350.0295.01
TCGCCTA1200.0295.02
GCCCCTA950.0295.02
GTTTGTC108.448463E-4295.0295
GTGCATC206.144728E-8295.0295
GCCCCCT206.144728E-8295.01
ATCTCCT350.0295.01
GTCGCCT303.6379788E-12295.01
AGATCCT450.0295.01
CGAACCT255.2568794E-10295.01
GTCGATA108.448463E-4295.0295
TCACCCT303.6379788E-12295.01
ATCTACG108.448463E-4295.04
CTATCCT350.0295.01
CGTGCCT255.2568794E-10295.01
GAGTCCT157.2120565E-6295.01
TGGACCT450.0295.01
CCGGCCT157.2120565E-6295.01
Produced by FastQC (version 0.11.5)