EGAF00005492233 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005492233
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40891
Sequences flagged as poor quality	0
Sequence length	301
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	58	0.14184050280012717	No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	58	0.14184050280012717	No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	57	0.13939497688978014	No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	54	0.1320583991587391	No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	54	0.1320583991587391	No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	53	0.12961287324839207	No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	53	0.12961287324839207	No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	52	0.12716734733804502	No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	52	0.12716734733804502	No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	52	0.12716734733804502	No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	51	0.12472182142769803	No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	50	0.122276295517351	No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	50	0.122276295517351	No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	49	0.11983076960700399	No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	49	0.11983076960700399	No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	49	0.11983076960700399	No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	49	0.11983076960700399	No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	48	0.11738524369665697	No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	48	0.11738524369665697	No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	48	0.11738524369665697	No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	47	0.11493971778630994	No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	47	0.11493971778630994	No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	47	0.11493971778630994	No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	47	0.11493971778630994	No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	46	0.11249419187596293	No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	46	0.11249419187596293	No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	46	0.11249419187596293	No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	45	0.11004866596561591	No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	45	0.11004866596561591	No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	45	0.11004866596561591	No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	45	0.11004866596561591	No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	45	0.11004866596561591	No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	45	0.11004866596561591	No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	45	0.11004866596561591	No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	45	0.11004866596561591	No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	45	0.11004866596561591	No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	45	0.11004866596561591	No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	44	0.10760314005526887	No Hit
GACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	44	0.10760314005526887	No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	44	0.10760314005526887	No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	44	0.10760314005526887	No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	44	0.10760314005526887	No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	44	0.10760314005526887	No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	44	0.10760314005526887	No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	43	0.10515761414492186	No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	43	0.10515761414492186	No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	43	0.10515761414492186	No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	43	0.10515761414492186	No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	43	0.10515761414492186	No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	43	0.10515761414492186	No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	43	0.10515761414492186	No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	43	0.10515761414492186	No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	43	0.10515761414492186	No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	43	0.10515761414492186	No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	42	0.10271208823457484	No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	42	0.10271208823457484	No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	42	0.10271208823457484	No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	42	0.10271208823457484	No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	42	0.10271208823457484	No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	42	0.10271208823457484	No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	42	0.10271208823457484	No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	41	0.10026656232422783	No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	41	0.10026656232422783	No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	41	0.10026656232422783	No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	41	0.10026656232422783	No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	41	0.10026656232422783	No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	41	0.10026656232422783	No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC	41	0.10026656232422783	No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC	41	0.10026656232422783	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCCTA	35	0.0	295.00003	2
GAGCCTA	35	0.0	295.00003	2
TACACCT	35	0.0	295.00003	1
GATCCTA	35	0.0	295.00003	2
GCGCCTA	35	0.0	295.00003	2
CTACCTA	70	0.0	295.00003	2
CGTCCTA	70	0.0	295.00003	2
GGGCCTA	35	0.0	295.00003	2
ATCCCCT	10	8.4352214E-4	295.0	1
ACAGCCT	25	5.2204996E-10	295.0	1
GTTGATT	45	0.0	295.0	295
TGGTCCT	15	7.189461E-6	295.0	1
TCCTCGG	15	7.189461E-6	295.0	4
ACGCCTA	40	0.0	295.0	2
TGCACCT	20	6.112532E-8	295.0	1
CCAACCT	15	7.189461E-6	295.0	1
CCTACGA	20	6.112532E-8	295.0	5
GTCACCT	10	8.4352214E-4	295.0	1
ATCTCCT	20	6.112532E-8	295.0	1
AGCTCCT	25	5.2204996E-10	295.0	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content