FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45910
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC730.1590067523415378No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.15029405358309736No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC690.15029405358309736No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.14593770420387717No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC670.14593770420387717No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC650.14158135482465695No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC640.13940318013504685No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC640.13940318013504685No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.13722500544543673No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.13722500544543673No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC630.13722500544543673No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC630.13722500544543673No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC620.13504683075582663No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.1328686560662165No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC610.1328686560662165No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC600.1306904813766064No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC600.1306904813766064No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC600.1306904813766064No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC600.1306904813766064No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC590.12851230668699629No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC590.12851230668699629No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC590.12851230668699629No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC580.1263341319973862No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC570.12415595730777608No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC570.12415595730777608No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.12197778261816598No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.12197778261816598No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.12197778261816598No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.12197778261816598No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC560.12197778261816598No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC550.11979960792855587No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC550.11979960792855587No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC550.11979960792855587No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC540.11762143323894576No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC540.11762143323894576No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC540.11762143323894576No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC540.11762143323894576No Hit
GGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC540.11762143323894576No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC530.11544325854933567No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC530.11544325854933567No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC530.11544325854933567No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC530.11544325854933567No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.11326508385972554No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.11326508385972554No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.11326508385972554No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.11326508385972554No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.11326508385972554No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.11326508385972554No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC520.11326508385972554No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.11108690917011545No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.11108690917011545No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.11108690917011545No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.11108690917011545No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC510.11108690917011545No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC500.10890873448050534No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC500.10890873448050534No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC500.10890873448050534No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC500.10890873448050534No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC500.10890873448050534No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC500.10890873448050534No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
CTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC490.10673055979089523No Hit
TAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC480.10455238510128512No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC480.10455238510128512No Hit
GGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC480.10455238510128512No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC480.10455238510128512No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAAAGTC470.10237421041167502No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.10237421041167502No Hit
CAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.10237421041167502No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.10237421041167502No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.10237421041167502No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC470.10237421041167502No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC460.10019603572206492No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC460.10019603572206492No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC460.10019603572206492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT303.6379788E-12295.01
AGCCTAC2450.0295.03
TTGCATC157.1952563E-6295.0295
TTGTCCT303.6379788E-12295.01
TGGTCCT157.1952563E-6295.01
AATCCTA900.0295.02
TCCTCCT255.2386895E-10295.01
ACGCCTA500.0295.02
GTGTCGG850.0295.0295
CCAACCT255.2386895E-10295.01
GGGTCCT350.0295.01
AATCCCT206.120899E-8295.01
GTCACCT350.0295.01
CCTACCT157.1952563E-6295.01
GGCACCT157.1952563E-6295.01
AGCTCCT157.1952563E-6295.01
TATACCT450.0295.01
TTACCTA700.0295.02
TGACCTA900.0295.02
TCGACCT108.438619E-4295.01
Produced by FastQC (version 0.11.5)