FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67578
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10802332119920684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATT550.0295.00003295
GGGCCTA1100.0295.000032
TACTCCT550.0295.000031
TCGCCTA1000.0295.02
GTTGATC108.447431E-4295.0295
TCGCCCT255.2568794E-10295.01
GCCCCCT108.447431E-4295.01
GTGCAGA108.447431E-4295.0295
AATCCCT206.1421815E-8295.01
GATACCT108.447431E-4295.01
ATCTCCT255.2568794E-10295.01
AGTCCCT350.0295.01
TACCCCT350.0295.01
TGCCCCT500.0295.01
AGATCCT206.1421815E-8295.01
CGAACCT255.2568794E-10295.01
ACGCCTA850.0295.02
CCCTCCT255.2568794E-10295.01
TCACCCT350.0295.01
CACGGGA255.2568794E-10295.06
Produced by FastQC (version 0.11.5)