FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74942
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCT350.0295.000031
AGGACCT350.0295.000031
TTTTCCT350.0295.000031
CAATCCT350.0295.000031
CTGACCT350.0295.000031
CGGACCT350.0295.000031
TAGTCCT350.0295.000031
ACCCCTA1250.0295.000032
GAATCCT350.0295.000031
GCACCTA1400.0295.000032
GACCCCT350.0295.000031
CGCACCT350.0295.000031
CAGCCCT450.0295.01
CGTTCCT255.2568794E-10295.01
ATCCCCT303.6379788E-12295.01
ACAGCCT206.146547E-8295.01
AAACCTA1200.0295.02
TGGCATA108.4492646E-4295.0295
TGGTCCT157.213426E-6295.01
GTGTCGG400.0295.0295
Produced by FastQC (version 0.11.5)