FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110563
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC2060.18631911218038583No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1990.17998788021309117No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1900.17184772482656946No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1860.1682298779881154No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1840.16642095456888834No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1840.16642095456888834No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1800.16280310773043422No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1770.16008972260159365No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1760.15918526089198015No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1700.153758490634299No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1700.153758490634299No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1690.15285402892468547No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1690.15285402892468547No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1690.15285402892468547No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1680.15194956721507194No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1660.1501406437958449No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1660.1501406437958449No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1610.1456183352477773No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1600.14471387353816376No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1590.14380941182855025No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1580.1429049501189367No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1580.1429049501189367No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1560.14109602669970966No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1560.14109602669970966No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1550.14019156499009616No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1540.13928710328048263No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1540.13928710328048263No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1520.13747817986125557No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1520.13747817986125557No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1510.13657371815164204No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1510.13657371815164204No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1500.13566925644202854No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1500.13566925644202854No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1500.13566925644202854No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1490.134764794732415No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1490.134764794732415No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1490.134764794732415No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1480.13386033302280148No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1480.13386033302280148No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1480.13386033302280148No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1480.13386033302280148No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1470.13295587131318795No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1450.13114694789396092No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1450.13114694789396092No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1450.13114694789396092No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1440.1302424861843474No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1440.1302424861843474No Hit
GAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1440.1302424861843474No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1440.1302424861843474No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1440.1302424861843474No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1430.12933802447473386No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1430.12933802447473386No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1420.12843356276512033No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1420.12843356276512033No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1420.12843356276512033No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1420.12843356276512033No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1420.12843356276512033No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1410.1275291010555068No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1410.1275291010555068No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1400.1266246393458933No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1400.1266246393458933No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1390.12572017763627977No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1390.12572017763627977No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1390.12572017763627977No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1390.12572017763627977No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1390.12572017763627977No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1380.12481571592666625No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1380.12481571592666625No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1380.12481571592666625No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1380.12481571592666625No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1380.12481571592666625No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1370.12391125421705271No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1370.12391125421705271No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1370.12391125421705271No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1350.12210233079782568No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1350.12210233079782568No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1350.12210233079782568No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1350.12210233079782568No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1350.12210233079782568No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1340.12119786908821216No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1340.12119786908821216No Hit
GTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1340.12119786908821216No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1340.12119786908821216No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1330.12029340737859863No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1330.12029340737859863No Hit
GTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1330.12029340737859863No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1320.11938894566898509No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1320.11938894566898509No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1320.11938894566898509No Hit
AGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1320.11938894566898509No Hit
TTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1320.11938894566898509No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1320.11938894566898509No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1310.11848448395937157No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1310.11848448395937157No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1310.11848448395937157No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1310.11848448395937157No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1310.11848448395937157No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1300.11758002224975805No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.11667556054014454No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.11667556054014454No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.11667556054014454No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.11667556054014454No Hit
ATTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.11667556054014454No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.11667556054014454No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1290.11667556054014454No Hit
AGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1280.11577109883053102No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1270.11486663712091749No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1260.11396217541130395No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1250.11305771370169043No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1250.11305771370169043No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1250.11305771370169043No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1250.11305771370169043No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1240.11215325199207692No Hit
GATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1230.1112487902824634No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1230.1112487902824634No Hit
GTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1230.1112487902824634No Hit
CATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1220.11034432857284987No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1210.10943986686323634No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1210.10943986686323634No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1200.10853540515362281No Hit
ACGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1200.10853540515362281No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1200.10853540515362281No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1200.10853540515362281No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1190.1076309434440093No Hit
TAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1190.1076309434440093No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1190.1076309434440093No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1190.1076309434440093No Hit
TTGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1180.10672648173439578No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1180.10672648173439578No Hit
TCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1180.10672648173439578No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1170.10582202002478226No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1170.10582202002478226No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1170.10582202002478226No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1170.10582202002478226No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1170.10582202002478226No Hit
GTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1170.10582202002478226No Hit
GCTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1040130966055552No Hit
CGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1040130966055552No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1040130966055552No Hit
AGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1040130966055552No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1040130966055552No Hit
CGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1150.1040130966055552No Hit
TAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.10310863489594167No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.10310863489594167No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.10310863489594167No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.10310863489594167No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1140.10310863489594167No Hit
CGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1130.10220417318632816No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1130.10220417318632816No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.10129971147671464No Hit
GGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.10129971147671464No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1120.10129971147671464No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.10039524976710112No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1110.10039524976710112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCCT550.0295.000031
CCGCCTA1100.0295.000032
TACGCCT550.0295.000031
GATCCTA2050.0295.000032
ATACCTA2200.0295.000032
CGTTCCT500.0295.01
ATCCCCT500.0295.01
ACGCCTA1550.0295.02
TGCACCT255.275069E-10295.01
GGGTCCT350.0295.01
CCTACCT450.0295.01
AGCTCCT350.0295.01
AAATCCT750.0295.01
CTCCCCT500.0295.01
AATTCCT500.0295.01
ACGTCCT350.0295.01
CAACCTA1750.0295.02
GGTCCTA2100.0295.02
GGCGCCT255.275069E-10295.01
CGGGGCA350.0295.09
Produced by FastQC (version 0.11.5)