FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77365
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA840.10857622956117108No Hit
TTCACCTACGGGAGGCAGCAGGGGTTAGGTATGACCTTGCTCCCAACCAA810.10469850707684354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT206.148002E-8295.01
TCGCCTA1150.0295.02
GCCCCTA1300.0295.02
ATCCTCG108.449799E-4295.03
GTCGCCT400.0295.01
TAGACCT303.6379788E-12295.01
CGGCCCT303.6379788E-12295.01
ACCGCCT108.449799E-4295.01
CGACCTA800.0295.02
GGATCCT255.2568794E-10295.01
GCGCCTA950.0295.02
CCGTCCT800.0295.01
CGCCTAG157.2143375E-6295.03
AAGCCTA1100.0295.02
TACGCCT450.0295.01
TGCGCCT550.0295.01
CTGCCTA800.0295.02
CCCCCTA1150.0295.02
GGACGTG108.449799E-4295.0295
CTACCTA1700.0295.02
Produced by FastQC (version 0.11.5)