FastQCFastQC Report
Wed 8 May 2024
EGAF00005492261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76616
Total Bases23 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG900.11746893599248198No Hit
TTCACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG830.10833246319306672No Hit
ATAACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG800.10441683199331733No Hit
TTCTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG770.10050120079356792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)