FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76616
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG900.11746893599248198No Hit
TTCACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG830.10833246319306672No Hit
ATAACCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG800.10441683199331733No Hit
TTCTCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG770.10050120079356792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCCTA950.0295.000032
GCCCCTA850.0295.02
GTCGCCT350.0295.01
CGAACCT400.0295.01
GGATCCT206.1474566E-8295.01
GCGCCCT350.0295.01
TACGCCT400.0295.01
CCCAACG108.449638E-4295.04
GAGCCTA350.0295.02
GACGCCT350.0295.01
GGCGCCT108.449638E-4295.01
CGCACCT400.0295.01
CCGCCTA650.0295.02
CCCGCCT206.1474566E-8295.01
ACGCCTA1200.0294.999972
ACGCCCT157.2140647E-6294.999971
AAGCCTA1050.0294.999972
CTCGCCT303.6379788E-12294.999971
CGTACCT303.6379788E-12294.999971
ATCGCCT303.6379788E-12294.999971
Produced by FastQC (version 0.11.5)