FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78831
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC870.11036267458233436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA1050.0295.02
GTTTGTC108.4500987E-4295.0295
AATCCCT157.2148505E-6295.01
CTGTCCT255.275069E-10295.01
AGATCCT157.2148505E-6295.01
GCGACCT206.14873E-8295.01
TGGACCT500.0295.01
CGGCCCT303.6379788E-12295.01
ACACCCT450.0295.01
GTCCTAG108.4500987E-4295.03
CGACCTA1150.0295.02
TACGGGC108.4500987E-4295.07
CGTTCCT303.6379788E-12295.01
ACGGGGC108.4500987E-4295.08
GACCCCT450.0295.01
GTTACCT600.0295.01
CGGGGCA108.4500987E-4295.09
TACGCCT255.275069E-10295.01
CGCTCCT400.0295.01
CTGCCTA600.0295.02
Produced by FastQC (version 0.11.5)