FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67573
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10951119529989789No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCTA1100.0295.000032
GCCCCTA700.0295.02
AATCCTA1250.0295.02
TACCCCT350.0295.01
CGAACCT108.447431E-4295.01
GTCGATA108.447431E-4295.0295
CGTGCCT108.447431E-4295.01
GATCCTA950.0295.02
GGTCCCT350.0295.01
CGCGCCT255.2568794E-10295.01
GGATCCT350.0295.01
TGAACCT206.1421815E-8295.01
GCGCCTA750.0295.02
TCCTCGG108.447431E-4295.04
ACGGGGC206.1421815E-8295.08
GACCCCT108.447431E-4295.01
GCACCTA800.0295.02
GCACCCT108.447431E-4295.01
GTTACCT400.0295.01
ATTCCCT108.447431E-4295.01
Produced by FastQC (version 0.11.5)