FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67573
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA24413.612389563879064No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA21853.23354002338212No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17942.654906545513741No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA15202.249419146700605No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10791.5967916179539163No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA10481.5509153064093646No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA10071.4902401846891509No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9781.4473236351797316No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA9431.3955277995649151No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA9211.3629704171784587No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA8321.2312610066150682No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA7671.1350687404732658No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA7581.1217498113151703No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA7331.0847527858760155No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6630.9811611146463826No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA6550.969322066505853No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6360.9412043271720953No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA6280.9293652790315657No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6250.9249256359788673No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6130.907167063768073No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5990.8864487295221465No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5580.8257736078019327No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA5390.7976558684681753No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5300.7843369393100794No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5140.7606588430290204No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA4770.7059032453790715No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4660.6896245541858435No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4510.6674263389223506No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA4450.6585470528169536No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4260.630429313483196No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3910.5786334778683794No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3830.5667944297278499No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA3830.5667944297278499No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3770.5579151436224528No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA3740.5534755005697543No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3740.5534755005697543No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA3630.5371968093765261No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA3510.5194382371657319No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3430.5075991890252023No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3340.4942802598671067No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3080.4558033534103858No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA3040.449883829340121No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2920.4321252571293268No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2870.4247258520414959No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2750.4069672798307016No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA2670.39512823169017214No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2530.37440989744424547No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA2530.37440989744424547No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2520.3729300164266793No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2510.37145013540911315No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA2500.3699702543915469No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2430.3596110872685836No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2420.3581312062510174No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2420.3581312062510174No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA2300.34037263404022317No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2300.34037263404022317No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2180.3226140618294289No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2060.3048554896186347No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2010.29745608453080374No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1960.2900566794429728No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1930.2856170363902742No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1910.2826572743551419No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1890.2796975123200095No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1840.27229810723217857No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1840.27229810723217857No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1800.26637858316191376No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1780.2634188211267814No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1780.2634188211267814No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1770.26193894010921526No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1680.24862001095111952No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1640.24270048688085477No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1610.2382608438281562No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1560.23086143874032528No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1550.22938155772275912No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1540.2279016767051929No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1490.22050227161736197No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1440.21310286652953103No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1420.21014310449439863No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1410.20866322347683247No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1400.20718334245926626No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1400.20718334245926626No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1380.2042235804241339No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1370.2027436994065677No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.19830405635386916No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1330.19682417533630295No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1290.19090465126603823No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1260.18646500821333964No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1250.18498512719577345No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1250.18498512719577345No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1210.1790656031255087No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1210.1790656031255087No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1210.1790656031255087No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.17758572210794252No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1200.17758572210794252No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA1200.17758572210794252No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1160.17166619803767777No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1140.1687064360025454No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.1672265549849792No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGA1130.1672265549849792No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1120.16574667396741302No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1110.16426679294984683No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1110.16426679294984683No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1060.15686738786201587No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA1030.15242774480931734No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA1020.15094786379175115No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1000.14798810175661875No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA990.1465082207390526No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA960.142068577686354No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA960.142068577686354No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA950.14058869666878782No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA940.13910881565122163No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA940.13910881565122163No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA880.1302295295458245No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA880.1302295295458245No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA870.12874964852825835No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA860.12726976751069213No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA850.12578988649312595No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA850.12578988649312595No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA840.12431000547555976No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG840.12431000547555976No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA810.11987036242286121No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA810.11987036242286121No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA810.11987036242286121No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA800.11839048140529503No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC780.11543071937016264No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCTGCACTTTCGAGCCTCA760.11247095733503025No Hit
GGACTACTAGGGTTTCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGA750.11099107631746409No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG750.11099107631746409No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCTGCACTTTCGAGCCTCA730.1080313142823317No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCTGCACTTTCGAGCCTCA720.10655143326476552No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC720.10655143326476552No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCA700.10359167122963313No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG700.10359167122963313No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)