FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63803
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA550.0295.000032
GCACCTA1100.0295.000032
GGACCTA1100.0295.000032
TCGCCCT206.139453E-8295.01
GTCGCCT108.4463326E-4295.01
CGAACCT108.4463326E-4295.01
TGGACCT255.2568794E-10295.01
ACCGCCT108.4463326E-4295.01
GTCCTAC3300.0295.03
GGTCCTA700.0295.02
TGGCGCT206.139453E-8295.0295
ATAGCCT108.4463326E-4295.01
GCGCCTA650.0295.02
GCGCCCT108.4463326E-4295.01
GTTACCT350.0295.01
GTAGCCT206.139453E-8295.01
TACGCCT400.0295.01
AGCGCCT108.4463326E-4295.01
TTGCCTA650.0295.02
GTCCCCT206.139453E-8295.01
Produced by FastQC (version 0.11.5)