FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110785
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1470.13268944351672157No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1430.12907884641422576No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1410.12727354786297784No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1400.1263708985873539No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1390.12546824931172992No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1380.12456560003610596No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1360.12276030148485806No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1350.1218576522092341No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1350.1218576522092341No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1330.1200523536579862No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1330.1200523536579862No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1310.11824705510673829No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1310.11824705510673829No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1290.11644175655549037No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1290.11644175655549037No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1290.11644175655549037No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1280.1155391072798664No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1270.11463645800424245No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1270.11463645800424245No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1260.11373380872861849No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1260.11373380872861849No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1260.11373380872861849No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1260.11373380872861849No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1250.11283115945299453No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1250.11283115945299453No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1240.11192851017737059No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1230.11102586090174663No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1230.11102586090174663No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1220.11012321162612267No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1220.11012321162612267No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1220.11012321162612267No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1210.10922056235049872No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1200.10831791307487476No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1190.10741526379925082No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1190.10741526379925082No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1180.10651261452362686No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1180.10651261452362686No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1180.10651261452362686No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1180.10651261452362686No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1170.10560996524800288No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1170.10560996524800288No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1170.10560996524800288No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1170.10560996524800288No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1150.10380466669675498No Hit
GGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1150.10380466669675498No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1140.10290201742113102No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1130.10199936814550706No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1130.10199936814550706No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1130.10199936814550706No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1130.10199936814550706No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1130.10199936814550706No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1120.10109671886988311No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1120.10109671886988311No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1120.10109671886988311No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1120.10109671886988311No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1110.10019406959425915No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1110.10019406959425915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCT550.0295.000031
TCGCCTA1300.0295.02
GCCCCTA1200.0295.02
GCCCCCT206.159826E-8295.01
AGATCCT350.0295.01
GGTCCCT305.456968E-12295.01
CCTACGA206.159826E-8295.05
GTTAGTA108.454721E-4295.0295
GCGCCCT255.275069E-10295.01
CGTTCCT650.0295.01
GACCCCT206.159826E-8295.01
GTAGCCT350.0295.01
TACGCCT450.0295.01
CTACGAG206.159826E-8295.06
GGGCGGC108.454721E-4295.09
CGTACCT650.0295.01
CGAGCCT206.159826E-8295.01
CCGACCT255.275069E-10295.01
GACGCCT206.159826E-8295.01
GGCGCCT157.222745E-6295.01
Produced by FastQC (version 0.11.5)