FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82422
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1010.12254009851738613No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.1116206837980151No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC910.11040741549586275No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.1079808788915581No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.1055543422872534No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.1055543422872534No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.10434107398510106No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.10434107398510106No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.10312780568294874No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.10312780568294874No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.1019145373807964No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.10070126907864405No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.10070126907864405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCTA850.0295.000032
GTTGATT108.4507983E-4295.0295
TCTGCCT157.2160456E-6295.01
GCCCCTA800.0295.02
GAGTCCT350.0295.01
TGGACCT206.150367E-8295.01
CCGGCCT400.0295.01
TTGTCCT650.0295.01
CCTACCT450.0295.01
GCGCCCT650.0295.01
CGTTCCT350.0295.01
AGAACCT450.0295.01
GTTACCT206.150367E-8295.01
AGGCCTA950.0295.02
GTAGCCT303.6379788E-12295.01
AAGCCCT303.6379788E-12295.01
GCTTACG108.4507983E-4295.04
TCGTCCT400.0295.01
GAGCCCT157.2160456E-6295.01
GCCTAAG206.150367E-8295.04
Produced by FastQC (version 0.11.5)