FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90938
Sequences flagged as poor quality0
Sequence length301
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG950.10446677956409862No Hit
CCATCCTACGGGAGGCAGCAGGACTAGGGGGTTGGGGACAGATTTTATTG920.1011678286304955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT450.0295.01
GTCGCCT255.275069E-10295.01
GGTCCTA900.0295.02
CGCGCCT255.275069E-10295.01
GCGCCTA750.0295.02
CGTTCCT255.275069E-10295.01
ACCTCCT500.0295.01
TCTACCT350.0295.01
GAGCCTA650.0295.02
CCGACCT500.0295.01
TAAGCCT450.0295.01
GCGTCCT350.0295.01
CCCCTCG108.452232E-4294.999973
CAACGGG108.452232E-4294.999976
ACGGGGC157.218494E-6294.999978
GACCCCT108.452232E-4294.999971
TGCGCCT108.452232E-4294.999971
CTCGCCT303.6379788E-12294.999971
CGGTCCT206.153823E-8294.999971
GGCGCCT303.6379788E-12294.999971
Produced by FastQC (version 0.11.5)