FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98071
Sequences flagged as poor quality0
Sequence length301
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT400.0295.01
TCGCCTA1300.0295.02
TCGCCCT157.220222E-6295.01
GTCGCCT450.0295.01
TGCCCCT157.220222E-6295.01
GCGACCT255.275069E-10295.01
GCGCCCT157.220222E-6295.01
TACGCCT305.456968E-12295.01
CGCTACG157.220222E-6295.03
CGTACCT350.0295.01
TCCCGGA108.4532436E-4295.03
GTCCCCT157.220222E-6295.01
CCGCATT108.4532436E-4295.0295
ATCGCCT305.456968E-12295.01
CTTACGG157.220222E-6295.05
GTTGCCT108.4532436E-4295.01
GGCCTAC4700.0291.86173
TCCTACG27250.0290.128454
CCTACGG92900.0289.919285
GACCTAC5750.0289.869573
Produced by FastQC (version 0.11.5)