FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219583
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAACCCTAGTAGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2380.10838726130893558Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCT600.0294.995241
GCGCCCT700.0294.995241
GGGCCCT650.0294.99521
CCTACGG197900.0289.7785
CTACGGG187550.0289.17556
GGGCCTA2150.0288.134862
TACGGGA180500.0287.640787
CCCTACG46250.0286.384524
GCCTACG40550.0285.53794
TCCTACG54550.0285.53164
ACGGGAG181600.0285.492348
CGGGAGG174600.0285.280339
ACCTACG61450.0281.793644
GCGCCTA2800.0279.19192
GCCCCTA3550.0278.375762
TGGTCCT800.0276.5581
CTACGGT6950.0275.89486
GCCCTAC10750.0274.414183
CGCCTAC11750.0273.655123
TAGGCCT1300.0272.303281
Produced by FastQC (version 0.11.5)