EGAF00005492302 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005492302
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219583
Sequences flagged as poor quality	0
Sequence length	301
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per tile quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1312	0.5974961631820314	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1101	0.5014049357190675	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	1097	0.49958330107521987	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	914	0.4162435161191896	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	855	0.3893744051224366	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	852	0.3880081791395509	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	837	0.38117704922512213	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	767	0.34929844295778817	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	749	0.3411010870604737	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	711	0.323795557943921	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	671	0.3055792115054444	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	666	0.3033021682006348	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	649	0.2955602209642823	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	613	0.2791655091696534	No Hit
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	612	0.27871010050869144	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	608	0.2768884658648438	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	595	0.270968153272339	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	590	0.2686911099675294	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	581	0.26459243201887217	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	559	0.25457344147771005	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	535	0.24364363361462407	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	520	0.23681250370019535	No Hit
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	504	0.22952596512480472	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	498	0.22679351315903323	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	492	0.2240610611932618	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	481	0.21905156592268074	No Hit
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	479	0.2181407486007569	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	479	0.2181407486007569	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	478	0.217685339939795	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	476	0.21677452261787117	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTAGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	467	0.21267584466921394	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	463	0.21085421002536628	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	447	0.20356767144997565	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	438	0.19946899350131841	No Hit
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	433	0.19719195019650884	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	429	0.19537031555266118	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	425	0.19354868090881353	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCTGCC	423	0.1926378635868897	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	420	0.19127163760400395	No Hit
GGACTACTCGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	418	0.19036082028208012	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACTCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	418	0.19036082028208012	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	418	0.19036082028208012	No Hit
GGACTACTCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	412	0.18762836831630864	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	408	0.185806733672461	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACAAGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	404	0.18398509902861335	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	382	0.17396610848745123	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	379	0.17259988250456548	No Hit
GGACTACTAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	372	0.16941202187783208	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	371	0.16895661321687017	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	371	0.16895661321687017	No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	358	0.16303630062436528	No Hit
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	357	0.16258089196340336	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA	356	0.16212548330244145	No Hit
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	352	0.1603038486585938	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	350	0.15939303133666996	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	344	0.15666057937089847	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	334	0.15210649276127933	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	332	0.1511956754393555	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	327	0.14891863213454593	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	323	0.14709699749069827	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTAGGGTATCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	322	0.14664158882973635	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACCGGGGTTTCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	314	0.14299831954204104	RNA PCR Primer, Index 13 (96% over 28bp)
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA	308	0.14026586757626955	No Hit
GGACTACCCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	305	0.1388996415933838	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	301	0.13707800694953617	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA	289	0.1316131030179932	No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	286	0.13024687703510746	No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	285	0.12979146837414554	No Hit
GGACTACTAGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	285	0.12979146837414554	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	282	0.1284252423912598	No Hit
GGACTACAAGGGTATCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	279	0.12705901640837405	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACACGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	279	0.12705901640837405	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA	276	0.1256927904254883	No Hit
GGACTACCCGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	268	0.122049521137793	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCA	267	0.12159411247683108	No Hit
GGACTACAAGGGTTTCTCAAGCAGAAGACGGCATACGAGATTGAGGAGTG	265	0.12068329515490725	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA	263	0.11977247783298342	No Hit
GGACTACTAGGGTTTCTCAAGCAGAAGACGGCATACGAGATTGAGGAGTG	263	0.11977247783298342	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACCGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGGCTGCCTC	262	0.1193170691720215	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACAAGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	258	0.11749543452817385	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACTCGGGTATCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	254	0.11567379988432619	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACTCGGGTTTCTAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA	249	0.11339675657951662	Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTACACGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	249	0.11339675657951662	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGTGCTGCCT	244	0.11111971327470707	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACCCGGGTTTCTAATCAAGCAGAAGACGGCATACGAGATTGAGGA	241	0.10975348729182133	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACCGGGGTATCTAATCTCGTATGCCGTCTTCTGCTTGCTGCCTCC	240	0.10929807863085941	Illumina PCR Primer Index 12 (96% over 28bp)
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA	240	0.10929807863085941	No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA	232	0.1056548093431641	No Hit
GGACTACTCGGGTTTCTCAAGCAGAAGACGGCATACGAGATTGAGGAGTG	223	0.10155613139450687	RNA PCR Primer, Index 32 (96% over 28bp)
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA	222	0.10110072273354495	No Hit

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content