FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64513
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA900.0295.02
GTACCCT206.139999E-8295.01
TGCCCTA450.0295.02
CGCGCCT206.139999E-8295.01
GCGCCCT157.208784E-6295.01
CGTTCCT157.208784E-6295.01
CGGGGCA157.208784E-6295.09
GTGTCGG303.6379788E-12295.0295
TATTCCT206.139999E-8295.01
GAAACCT108.446547E-4295.01
CTCGCCT206.139999E-8295.01
CGAGCCT255.2568794E-10295.01
CCGACCT255.2568794E-10295.01
TTGCCTA850.0295.02
GACGCCT108.446547E-4295.01
GGCGCCT206.139999E-8295.01
CGCACCT206.139999E-8295.01
CGTCCTA1200.0295.02
GATTCCT303.6379788E-12295.01
TATACCT400.0295.01
Produced by FastQC (version 0.11.5)