FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72693
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1020.14031612397342247No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1010.13894047569917325No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC1000.137564827424924No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.1348135308764255No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC980.1348135308764255No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.13343788260217626No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.13343788260217626No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC960.13206223432792702No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC960.13206223432792702No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC940.12931093777942856No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC920.12655964123093008No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12518399295668084No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12380834468243158No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12380834468243158No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12380834468243158No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC900.12380834468243158No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12243269640818234No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12243269640818234No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC890.12243269640818234No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12105704813393313No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12105704813393313No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC880.12105704813393313No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC880.12105704813393313No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.11968139985968389No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.11968139985968389No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.11968139985968389No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.11968139985968389No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC860.11830575158543463No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC860.11830575158543463No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11830575158543463No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC860.11830575158543463No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC850.11693010331118539No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11693010331118539No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC850.11693010331118539No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11555445503693615No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11417880676268691No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11417880676268691No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11417880676268691No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11417880676268691No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11417880676268691No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11417880676268691No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11417880676268691No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC830.11417880676268691No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11417880676268691No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC830.11417880676268691No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.1128031584884377No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.1128031584884377No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC820.1128031584884377No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.1128031584884377No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC820.1128031584884377No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11142751021418844No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC810.11142751021418844No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11142751021418844No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11142751021418844No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.11142751021418844No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.1100518619399392No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.1100518619399392No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.1100518619399392No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC800.1100518619399392No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.1100518619399392No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10867621366568996No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10867621366568996No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC790.10867621366568996No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10730056539144071No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10730056539144071No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC780.10730056539144071No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC780.10730056539144071No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10730056539144071No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC780.10730056539144071No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10592491711719147No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10592491711719147No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC770.10592491711719147No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC770.10592491711719147No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10592491711719147No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC770.10592491711719147No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10454926884294223No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.10454926884294223No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10454926884294223No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10454926884294223No Hit
AAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10454926884294223No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC760.10454926884294223No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10454926884294223No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10454926884294223No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10454926884294223No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.103173620568693No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.103173620568693No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC750.103173620568693No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC740.10179797229444376No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC740.10179797229444376No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10179797229444376No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10179797229444376No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC740.10179797229444376No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10179797229444376No Hit
GGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC740.10179797229444376No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC740.10179797229444376No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC730.10042232402019452No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGGAGCC730.10042232402019452No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10042232402019452No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10042232402019452No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10042232402019452No Hit
CCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10042232402019452No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10042232402019452No Hit
TGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC730.10042232402019452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCT350.0295.000031
TGCACCT350.0295.000031
TGACCTA1100.0295.000032
GATACCT350.0295.000031
GGACCTA1100.0295.000032
GTTCCCT350.0295.000031
AACCCCT550.0295.000031
GTCCCCT350.0295.000031
CTTACCT350.0295.000031
ACGACCT350.0295.000031
GCACCCT350.0295.000031
CGCACCT550.0295.000031
ACAACCT350.0295.000031
GTGCCTA550.0295.000032
AGCCCTA550.0295.000032
TTGTCCT255.2568794E-10295.01
ACGCCTA950.0295.02
CCTACGA255.2568794E-10295.05
GTCACCT450.0295.01
CTCCCTA900.0295.02
Produced by FastQC (version 0.11.5)