FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72693
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17742.4404000385181517No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA16112.2161693698155256No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA14191.9520449011596714No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA13961.920404990851939No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13281.8268609082029905No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA13101.8020992392665043No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12741.7525759013935316No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA12071.6604074670188325No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11991.6494022808248385No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA11241.5462286602561457No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA11041.5187156947711609No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA11001.513213101674164No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA10551.4513089293329482No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA10401.4306742052192094No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA10341.422420315573714No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10201.4031612397342248No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9971.3715213294264923No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9691.3330031777475135No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9581.317871046730772No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9421.295860674342784No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA9081.24908863301831No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9071.2477129847440604No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA9051.244961688195562No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8981.2353321502758174No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA8701.1968139985968387No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA8681.19406270204834No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA8231.1321585297071244No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7941.0922647297538965No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7851.0798838952856533No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7651.0523709298006687No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7571.0413657436066748No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7381.015228426395939No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7240.9959693505564496No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA7190.9890911091852035No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA7150.9835885160882065No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6790.9340651782152339No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6690.9203086954727416No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6580.9051765644559999No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6560.9024252679075015No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6250.8597801714057749No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA6050.8322672059207902No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6010.8267646128237932No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5980.8226376680010454No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5460.751103957740085No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5300.7290935853520972No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA5260.7235909922551003No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5220.7180883991581033No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4900.6740676543821276No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4720.6493059854456412No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4560.6272956130576535No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4550.6259199647834042No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA4330.5956557027499209No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA4090.5626401441679392No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACTTTCGAGCCTCA3960.544756716602699No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3650.5021116201009727No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3500.48147689598723403No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3460.475974302890237No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3280.4512126339537508No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3250.447085689131003No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA3170.436080502937009No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2670.3672980892245471No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2660.36592244095029786No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2590.3562929030305531No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2550.3507903099335562No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2510.34528771683655923No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2350.32327734444857137No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2290.315023454803076No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2260.31089650998032825No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2210.30401826860908204No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2180.2998913237863343No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2170.298515675512085No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2090.28751048931809114No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA2090.28751048931809114No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA2030.27925659967259575No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1900.2613731721073556No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1750.24073844799361702No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1750.24073844799361702No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1710.23523585489662002No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1650.22698196525112457No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1630.22423066870262612No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1600.2201037238798784No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1540.21184983423438294No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1520.20909853768588446No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1480.20359594458888752No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1430.19671770321764132No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1380.1898394618463951No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1350.18571251702364738No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1330.18296122047514893No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1260.17333168255540424No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA1180.1623264963614103No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1090.14994566189316716No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1080.14857001361891792No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.14857001361891792No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1040.14306742052192095No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1040.14306742052192095No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1010.13894047569917325No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1000.137564827424924No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1000.137564827424924No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA960.13206223432792702No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA930.12793528950517932No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA920.12655964123093008No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA890.12243269640818234No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA890.12243269640818234No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA880.12105704813393313No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA870.11968139985968389No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA850.11693010331118539No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA840.11555445503693615No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA840.11555445503693615No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA840.11555445503693615No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.11417880676268691No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA820.1128031584884377No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA810.11142751021418844No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.1100518619399392No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.10730056539144071No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA780.10730056539144071No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA780.10730056539144071No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA770.10592491711719147No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA760.10454926884294223No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA750.103173620568693No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA750.103173620568693No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA740.10179797229444376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTT2800.0295.000038
ACTACTA5850.0295.000033
ATTTGCC700.0295.00003295
TGACTAC350.0295.000031
TTAGCCC450.0295.0295
CGTTAGC900.0295.0295
AGGGGTA3650.0295.08
GATACTA108.4487436E-4294.999972
TAGCCGG108.4487436E-4294.99997295
AGTTAGA157.212535E-6294.99997295
AGTTAAC108.4487436E-4294.99997295
TACTACT157.212535E-6294.999972
GGTCTAC206.145274E-8294.999971
AGTAGCC108.4487436E-4294.99997295
CTTAGCC108.4487436E-4294.99997295
AGTACGA108.4487436E-4294.99997295
GGTAGCC157.212535E-6294.99997295
TACCGGT108.4487436E-4294.999975
GGCTACA600.0294.999971
GGACTAC67600.0294.78181
Produced by FastQC (version 0.11.5)