FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22390
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCT206.05105E-8295.01
ACAGCCT108.409677E-4295.01
GAGGCCT108.409677E-4295.01
TTGTCCT108.409677E-4295.01
GTCACCT206.05105E-8295.01
TTACCTA550.0295.02
TGACCTA500.0295.02
CGCCCTA255.14774E-10295.02
TTCGCCT108.409677E-4295.01
TTACCCT108.409677E-4295.01
AGGACCT108.409677E-4295.01
GTTCCTA206.05105E-8295.02
GGCGCCT108.409677E-4295.01
CATCCCT108.409677E-4295.01
ACACCTA255.14774E-10295.02
GAGCCTA206.05105E-8295.02
ATTACCT206.05105E-8295.01
TCAGCCT108.409677E-4295.01
TTCCTAC1350.0295.03
TACGCCT108.409677E-4295.01
Produced by FastQC (version 0.11.5)