FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54181
Sequences flagged as poor quality0
Sequence length301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.1568815636477732No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC850.1568815636477732No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.15319023273841384No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC810.14949890182905445No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC800.14765323637437477No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC750.13842490910097635No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.134733578191617No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.1310422472822576No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.1310422472822576No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.12735091637289825No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.12735091637289825No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.12735091637289825No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.12550525091821857No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.12365958546353888No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.12365958546353888No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.12365958546353888No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.12365958546353888No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC660.1218139200088592No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC660.1218139200088592No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC660.1218139200088592No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC660.1218139200088592No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1199682545541795No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC640.11812258909949983No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC640.11812258909949983No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC630.11627692364482015No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC630.11627692364482015No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC630.11627692364482015No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC630.11627692364482015No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC630.11627692364482015No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC630.11627692364482015No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC630.11627692364482015No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC630.11627692364482015No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC620.11443125819014047No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC620.11443125819014047No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC620.11443125819014047No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC620.11443125819014047No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC620.11443125819014047No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC620.11443125819014047No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC620.11443125819014047No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC610.11258559273546076No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC610.11258559273546076No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC610.11258559273546076No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC610.11258559273546076No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC610.11258559273546076No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC610.11258559273546076No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC610.11258559273546076No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC600.11073992728078108No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC600.11073992728078108No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC600.11073992728078108No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC600.11073992728078108No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC590.1088942618261014No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC590.1088942618261014No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC590.1088942618261014No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC590.1088942618261014No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC580.10704859637142171No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC570.10520293091674203No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC570.10520293091674203No Hit
ATGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC570.10520293091674203No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC570.10520293091674203No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC570.10520293091674203No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC570.10520293091674203No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC560.10335726546206235No Hit
TAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC560.10335726546206235No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC560.10335726546206235No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC560.10335726546206235No Hit
AATCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC560.10335726546206235No Hit
CCGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC550.10151160000738266No Hit
GATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC550.10151160000738266No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC550.10151160000738266No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC550.10151160000738266No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC550.10151160000738266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA550.0295.000032
TGATCCT206.130904E-8295.01
TCGCCTA1050.0295.02
AGATCCT500.0295.01
CGAACCT206.130904E-8295.01
AGCCCTA500.0295.02
GAGTCCT400.0295.01
CGGCCCT108.442807E-4295.01
GTCCTAC3050.0295.03
GATCCTA1150.0295.02
GGTCCTA700.0295.02
GTCTCGT108.442807E-4295.0295
GCGCCTA750.0295.02
GCGCCCT255.2386895E-10295.01
TACGGAA108.442807E-4295.07
CCTCGGG450.0295.05
CGTTCCT108.442807E-4295.01
ACGGGGC108.442807E-4295.08
ACGGGCA108.442807E-4295.08
ACCTCGG206.130904E-8295.04
Produced by FastQC (version 0.11.5)