FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005492319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62686
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1060.1690967680183773No Hit
TCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT1020.1627157579044763No Hit
ACAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT970.1547394952621No Hit
TTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT970.1547394952621No Hit
ATATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.15154899020514948No Hit
TCTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.15154899020514948No Hit
TCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT950.15154899020514948No Hit
TAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.14995373767667422No Hit
ATCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT940.14995373767667422No Hit
CAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT920.14676323261972368No Hit
TTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.14516798009124846No Hit
ATCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT910.14516798009124846No Hit
CTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT900.1435727275627732No Hit
ACCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT900.1435727275627732No Hit
TTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.1387869699773474No Hit
TAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.1387869699773474No Hit
CCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT870.1387869699773474No Hit
TTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.1355964649203969No Hit
TTAGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT850.1355964649203969No Hit
TTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT840.13400121239192164No Hit
ATGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT840.13400121239192164No Hit
TTCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT840.13400121239192164No Hit
CTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT830.1324059598634464No Hit
TACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT830.1324059598634464No Hit
CCATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.13081070733497113No Hit
TACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.13081070733497113No Hit
GACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.13081070733497113No Hit
CACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT820.13081070733497113No Hit
CTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT810.12921545480649588No Hit
CATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT810.12921545480649588No Hit
TTTCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.1276202022780206No Hit
ACTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.1276202022780206No Hit
TTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.1276202022780206No Hit
ATAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT800.1276202022780206No Hit
AAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT780.1244296972210701No Hit
ATGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT780.1244296972210701No Hit
AATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
GTCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
TGGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
ATTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
CTGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
GTTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
AACTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
TGCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
CATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT770.12283444469259484No Hit
AAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT760.12123919216411957No Hit
AGCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT760.12123919216411957No Hit
TTGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT760.12123919216411957No Hit
CTCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.11964393963564432No Hit
CAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.11964393963564432No Hit
CTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT750.11964393963564432No Hit
TCGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11804868710716906No Hit
AACGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11804868710716906No Hit
ACTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11804868710716906No Hit
GTATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT740.11804868710716906No Hit
TCTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT730.1164534345786938No Hit
GGTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11485818205021855No Hit
TCCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11485818205021855No Hit
TGCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11485818205021855No Hit
CTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11485818205021855No Hit
GTACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11485818205021855No Hit
AAACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT720.11485818205021855No Hit
TATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.1132629295217433No Hit
TCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.1132629295217433No Hit
TCACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.1132629295217433No Hit
TGGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.1132629295217433No Hit
AATCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT710.1132629295217433No Hit
AACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
GTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
GAATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
TTGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
CCTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
ACGCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
CAGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
CCACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
GGTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT700.11166767699326803No Hit
CTTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT690.11007242446479278No Hit
AAGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT690.11007242446479278No Hit
ACCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT690.11007242446479278No Hit
ATTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT690.11007242446479278No Hit
AGGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT690.11007242446479278No Hit
CCCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10847717193631752No Hit
TAGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10847717193631752No Hit
TATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10847717193631752No Hit
GGATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT680.10847717193631752No Hit
GTAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10688191940784225No Hit
ATACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10688191940784225No Hit
CGTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10688191940784225No Hit
GATACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10688191940784225No Hit
TCCGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10688191940784225No Hit
GTGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT670.10688191940784225No Hit
TGTACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10528666687936701No Hit
AGATCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10528666687936701No Hit
ACGTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10528666687936701No Hit
CCCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT660.10528666687936701No Hit
TGCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
TACGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
ATGCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
CCTGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
TAGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
ACACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
CGCACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
ACCTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
AGTTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT650.10369141435089176No Hit
ACGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
CCCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
GCAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
TAGGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
TGAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
CACACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
GGGACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
GTGCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
GATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
CAACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
AGCCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT640.10209616182241649No Hit
GAACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10050090929394123No Hit
TGGGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10050090929394123No Hit
GGAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10050090929394123No Hit
CAAGCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10050090929394123No Hit
AATTCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10050090929394123No Hit
GCACCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10050090929394123No Hit
GAAACCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10050090929394123No Hit
AAGCCCTACGGGAGGCAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT630.10050090929394123No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)