FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76471
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC970.12684547083208014No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10330713603849825No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC790.10330713603849825No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10069176550587804No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC770.10069176550587804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCTA1100.0295.000032
GTTCCTA1100.0295.000032
GTCCCTA950.0295.000032
GTGCCTA550.0295.000032
GCCCCTA400.0295.02
GCCCCCT206.1474566E-8295.01
CGAACCT206.1474566E-8295.01
CCGGCCT255.2568794E-10295.01
ACCGCCT255.2568794E-10295.01
GATCCTA1350.0295.02
CACGCCT206.1474566E-8295.01
CGCGCCT206.1474566E-8295.01
ATTCCCT255.2568794E-10295.01
GTAGCCT108.449606E-4295.01
CGCTCCT255.2568794E-10295.01
GAAACCT255.2568794E-10295.01
TATTATC108.449606E-4295.0295
TCGTCCT400.0295.01
CGTACCT108.449606E-4295.01
CTACCCT350.0295.01
Produced by FastQC (version 0.11.5)