FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66847
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT350.0295.000031
TTAGCCT550.0295.000031
GCGCGAA350.0295.00003295
ATAGCCT350.0295.000031
AGCGCCT350.0295.000031
GCCCCTA1050.0295.02
CCCCTAC3550.0295.03
ACGCCTA850.0295.02
CCCTCGG206.141636E-8295.04
CGCGCCT255.2568794E-10295.01
GCGCCTA900.0295.02
GCGCCCT108.4472215E-4295.01
GCCGCCT206.141636E-8295.01
CGCCTAC3350.0295.03
TACGCCT206.141636E-8295.01
TCTACGG108.4472215E-4295.04
CGAGGCA108.4472215E-4295.08
CTCGCCT255.2568794E-10295.01
CCGACCT206.141636E-8295.01
ACGAGGC108.4472215E-4295.07
Produced by FastQC (version 0.11.5)