FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75227
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC920.12229651587860743No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12096720592340517No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC910.12096720592340517No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC870.11564996610259615No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC860.11432065614739388No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11166203623698939No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11166203623698939No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC840.11166203623698939No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC830.11033272628178713No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC820.10900341632658486No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.10767410637138261No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC810.10767410637138261No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10634479641618037No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC800.10634479641618037No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCC760.10102755659537135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA850.0295.000032
GTTGATT450.0295.0295
GCCCCTA800.0295.02
CGGAAGG108.449331E-4295.09
AGTCCTA1100.0295.02
AGATCCT206.146729E-8295.01
CGAACCT206.146729E-8295.01
GCGACCT206.146729E-8295.01
TCCGCCT400.0295.01
GGGCTAC108.449331E-4295.02
CACGGGA255.2568794E-10295.06
CTATCCT450.0295.01
TGGACCT550.0295.01
GTCCTAC4550.0295.03
GGTCCTA1250.0295.02
TTGTCCT255.2568794E-10295.01
CGACCTA1000.0295.02
TGAACCT400.0295.01
GCGCCTA800.0295.02
ACGGGAC108.449331E-4295.08
Produced by FastQC (version 0.11.5)