FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91497
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCT255.2568794E-10295.159761
GCACCTA1400.0295.159762
AACGCCT255.2568794E-10295.159761
GTACCCT400.0295.159731
GCGACCT303.6379788E-12295.159731
GCGCCTA800.0295.159732
CGCCTCG108.438615E-4295.159733
CGCTACG108.438615E-4295.159733
GTTCCCT303.6379788E-12295.159731
GACGCCT206.137452E-8295.159731
TTCGCCT400.0295.159731
ACCGGGA108.4524555E-4294.998358
CTACCGG303.6379788E-12294.998356
GCCTAGG206.154187E-8294.998354
GCTACGG206.154187E-8294.998354
GCCTACG17200.0289.853064
ACCTACG22900.0289.845554
CCTACGG85800.0289.325325
CCCTACG20800.0288.616184
CTACGGG84600.0288.547456
Produced by FastQC (version 0.11.5)