FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005492339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005492339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80207
Sequences flagged as poor quality0
Sequence length301
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCT350.0295.000031
TCGCCTA1000.0295.02
GCCCCTA950.0295.02
ACGCCTA900.0295.02
GCGCCTA750.0295.02
CGTTCCT450.0295.01
CGATCCT255.275069E-10295.01
ATCGCCT255.275069E-10295.01
GCGACCT157.215329E-6294.999971
CGCGCCT157.215329E-6294.999971
TACGGCG108.45038E-4294.999977
ACCTCGG157.215329E-6294.999974
ACGGCGG108.45038E-4294.999978
TCGCTTT108.45038E-4294.99997295
ACGACCT206.1492756E-8294.999971
ACCTACG20750.0293.57834
CCTACGG76300.0291.906925
GTCCTAC4650.0291.827943
CCCTACG18600.0291.034944
CTACGGG74250.0291.026956
Produced by FastQC (version 0.11.5)